Cloning and identification of a novel RNF6 transcrip-tional splice variant Spg2 in human development  

作　　者：ZHU Li TONG GuoQin CHEN Jie WANG Yun WANG SaiQun ZHAO MuZhi LI JianMin MA JianHua 

机构地区：[1]Lab of Reproductive Medicine,Department of Cell Biology and Medical Genetics,Nanjing Medical University,Nanjing 210029,China [2]Department of Endocrinology,Nanjing First Hospital,The Third Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China [3]Dapartment of Obstetrics and Gynecology,Nanjing Maternity and Child Health Care Hospital,Nanjing 210029,China

出　　处：《Science China(Life Sciences)》2008年第4期302-307,共6页中国科学（生命科学英文版）

基　　金：the National Basic Research Program(973 Program)of China(Grant No.G1999055901);National Natural Science Foundation of China(Grant No.30000088)

摘　　要：RING finger proteins are zinc finger proteins containing the RING motifs. They act mainly as E3 ubiq-uitin ligases, bind the ubiquitin E2 conjugating enzyme and promote degradation of targeted proteins, Many novel genes have been isolated and differentially expressed in human adult and embryo testis by a testis cDNA-array differential display technique. A novel RING finger cDNA is highly expressed in adult testis and at low level in fetal testis. It was named Spg2. It contains a 2055 nucleotide ORF, en-codes a 685-amino-acid RNF6 protein, and has a RING finger in its C terminal. NCBI Blast shows that the gene is located on chromosome 13 and contains five exons. A multiple tissue expression profile also indicates that it is highly expressed in human testis, so we speculate that it may be associated with human spermatogenesis by virtue of the action of its RING domain.RING finger proteins are zinc finger proteins containing the RING motifs. They act mainly as E3 ubiq-uitin ligases, bind the ubiquitin E2 conjugating enzyme and promote degradation of targeted proteins, Many novel genes have been isolated and differentially expressed in human adult and embryo testis by a testis cDNA-array differential display technique. A novel RING finger cDNA is highly expressed in adult testis and at low level in fetal testis. It was named Spg2. It contains a 2055 nucleotide ORF, en-codes a 685-amino-acid RNF6 protein, and has a RING finger in its C terminal. NCBI Blast shows that the gene is located on chromosome 13 and contains five exons. A multiple tissue expression profile also indicates that it is highly expressed in human testis, so we speculate that it may be associated with human spermatogenesis by virtue of the action of its RING domain.

关 键 词：alternative splice RING domain UBIQUITINATION SPERMATOGENESIS 

分 类 号：Q78[生物学—分子生物学]