Interactions between meso-tetrakis(4-(N-methylpyridiumyl))porphyrin TMPyP4 and DNA G-quadruplex of telomeric repeated sequence TTAGGG  

作　　者：ZHANG HuiJuan WANG XueFei WANG Peng PANG SiPing AI XiCheng ZHANG JianPing 

机构地区：[1]School of Life Science&Technology,Beijing Institute of Technology,Beijing 100081,China [2]Department of Chemistry,Renmin University of China,Beijing 100872,China [3]Institute of Chemistry,Chinese Academy of Sciences,Beijing 100080,China

出　　处：《Science China Chemistry》2008年第5期452-456,共5页中国科学（化学英文版）

基　　金：the National Natural Science Foundation of China (Grant Nos. 20442004, 10576002 and 20703067)

摘　　要：The binding properties between meso-tetrakis(4-(N-methylpyridiumyl))porphyrin (TMPyP4) and the parallel DNA G-quadruplex (G4) of telomeric repeated sequence 5′-TTAGGG-3′ have been characterized by means of circular dichroism,steady-state absorption,steady-state fluorescence and picosecond time-resolved fluorescence spectroscopies. The binding constant and the saturated binding number were determined as 1.29×106 (mol/L)-1 and 3,respectively,according to steady-state absorption spec-troscopy. Based on the findings by the use of time-resolved fluorescence spectroscopic technique,it is deduced that TMPyP4 binds to a DNA G-quadruplex with both the thread-intercalating and end-stacking modes and at the saturated binding state,one TMPyP4 molecule intercalates into the intervals of G-tetrads while the other two stack to the ends of the DNA G-quadruplex.The binding properties between meso-tetrakis(4-(N-methylpyridiumyl))porphyrin (TMPyP4) and the parallel DNA G-quadruplex (G4) of telomeric repeated sequence 5′-TTAGGG-3′ have been characterized by means of circular dichroism, steady-state absorption, steady-state fluorescence and picosecond time-resolved fluorescence spectroscopies. The binding constant and the saturated binding number were determined as 1.29×106 (mol/L)?1 and 3, respectively, according to steady-state absorption spectroscopy. Based on the findings by the use of time-resolved fluorescence spectroscopic technique, it is deduced that TMPyP4 binds to a DNA G-quadruplex with both the thread-intercalating and end-stacking modes and at the saturated binding state, one TMPyP4 molecule intercalates into the intervals of G-tetrads while the other two stack to the ends of the DNA G-quadruplex.

关 键 词：TMPyP4 DNA G-QUADRUPLEX SPECTROSCOPIC technique BINDING mode 

分 类 号：O631.3[理学—高分子化学]