乳粉中普通变形杆菌和奇异变形杆菌复合PCR-DHPLC检测技术的建立  

Identification of Proteus vulgaris and Proteus Mirabilis in milk Powders by Multiplex PCR-DHPLC

作　　者：曹际娟[1] 徐君怡[1] 孙哲平[1] 于珂[1] 郑秋月[1,2] 郑慧芳[3] 王秋艳[1] 

机构地区：[1]辽宁出入境检验检疫局,大连116001 [2]沈阳农业大学,沈阳110161 [3]云南出入境检验检疫局,昆明650228

出　　处：《生物技术通报》2008年第S1期420-424,共5页Biotechnology Bulletin

基　　金：国家“十一五”科技支撑计划课题“食品中微生物高通量检测试剂盒的研制”(课题编号:2006BAK02A13)的部分研究内容

摘　　要：应用复合PCR结合变性高效液相色谱(DHPLC)技术,建立乳粉中普通变形杆菌和奇异变形杆菌的高通量检测方法。根据普通变形杆菌的blaA和blaB基因及奇异变形杆菌的ureR基因序列分别设计特异性引物,复合PCR扩增的产物经DHPLC技术进行快速检测,并以37株参考菌株做特异性试验。试验结果表明,该方法具有很好的特异性,经复合PCR-DHPLC可同时检测乳粉中普通变形杆菌和奇异变形杆菌。该方法可以快速、准确、高通量检测普通变形杆菌和奇异变形杆菌,是乳粉中致病菌高通量检测的新技术。To identify Proteus vulgaris and Proteus mirabili in milk powders,a multiplex PCR-Denaturing High-Performance Liquid Chromatography(DHPLC)assay was performed in this study.Primers specific for blaA and blaB gene of Proteus vulgaris and ureR gene of Proteus mirabilis were selected to conduct the multiplex PCR-DHPLC assays.The specific testing was performed with 37 strains.The good specificity was found in the study.Also,it can detect and identify Proteus vulgaris and Proteus mirabilis quickly and highthrough by a multiplex PCR-DHPLC.The DHPLC could be a new method for detecting pathogens in milk powders.

关 键 词：复合PCR-DHPLC 乳粉 普通变形杆菌 奇异变形杆菌 

分 类 号：R155.5[医药卫生—营养与食品卫生学]