NanoLC-ESI MS/MS对重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白的准确鉴定  

Characterization of Recombinant Human Necrosis Factor-αReceptorⅡ:IgG Fc(rhTNFR:Fc) Fusion Protein by NanoUPLC-ESI MS/MS

作　　者：柏兆方[1] 李萍[1] 李卫华[1] 刘炳玉[1] 张学敏[1] 杨松成[1] 王红霞[1] 

机构地区：[1]军事医学科学院国家生物医学分析中心,北京100850

出　　处：《分析测试学报》2008年第S1期3-4,共2页Journal of Instrumental Analysis

摘　　要：NanoUPLC-ESI MS/MS was used to characterize the primary structure of recombinant human necrosis factor-αreceptorⅡ:IgG Fc fusion protein.The result indicated that the IgG Fc was IgG1 Fc but not others.This study is important for this recombinant protein medicine to enter into international market.NanoUPLC-ESI MS/MS was used to characterize the primary structure of recombinant human necrosis factor-αreceptorⅡ:IgG Fc fusion protein.The result indicated that the IgG Fc was IgG1 Fc but not others.This study is important for this recombinant protein medicine to enter into international market.

关 键 词：RECOMBINANT human NECROSIS factor-αreceptorⅡ:IgG Fc(rhTNFR:Fc) fusion PROTEIN nanoUPLC-ESI MS/MS 

分 类 号：R927[医药卫生—药学]