高效液相色谱-串联质谱法对猪肉中四种大环内酯类药物残留量的测定  

Determination of Four Macrolides in Pork by High Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：夏曦[1,2] 李晓薇[1,2] 江海洋[1,2] 李建成[1,2] 安保超 丁双阳[1,2] 

机构地区：[1]中国农业大学动物医学院,北京100193 [2]国家兽药残留基准实验室,北京100193

出　　处：《分析测试学报》2008年第S1期224-226,230,共4页Journal of Instrumental Analysis

基　　金：“十一五”国家科技支撑计划课题(2006BAK02A09)

摘　　要：A simple and sensitive method was developed for the simultaneous determination of four macrolides in pork using high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).Homogenized sample was extracted with NaH2PO4 buffer and acetonitrile solution,and defatted with n-hexane.Further cleanup was performed on a Sep-Pakt C18 solid-phase extraction cartridge.The compounds were determined by LC-MS/MS operated in positive electrospray ionization mode.The limits of detection(LODs) were 0.5 μg/kg.The average recoveries at three spiked concentration levels of 1.0,5.0,10.0 μg/kg were in the range of 70%-110%,with the intra-day RSD of less than 12.9% and inter-day RSD of less than 13.4%.A simple and sensitive method was developed for the simultaneous determination of four macrolides in pork using high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).Homogenized sample was extracted with NaH2PO4 buffer and acetonitrile solution,and defatted with n-hexane.Further cleanup was performed on a Sep-Pakt C18 solid-phase extraction cartridge.The compounds were determined by LC-MS/MS operated in positive electrospray ionization mode.The limits of detection(LODs) were 0.5 μg/kg.The average recoveries at three spiked concentration levels of 1.0,5.0,10.0 μg/kg were in the range of 70%-110%,with the intra-day RSD of less than 12.9% and inter-day RSD of less than 13.4%.

关 键 词：high performance liquid chromatography-tandem mass SPECTROMETRY PORK MACROLIDE 

分 类 号：R155.5[医药卫生—营养与食品卫生学]