Modeling of kiwifruit metallothionein kiwi503  

作　　者：Chunming Zhu Tun Lü Riqing Zhang Nanming Zhao Jinyuan Liu 

机构地区：[1]Tsing Hua Univ, State Key Lab Biomembrane & Membrane Biotechnol, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China

出　　处：《Chinese Science Bulletin》2000年第15期1413-1417,1444,共6页

摘　　要：Kiwifruit metallothionein, kiwi503, is a typical plant metallothionein protein. It has 63 amino acid residues in two cysteine-rich regions and one spacer region of about 32 residues. In this note, the two cysteine-rich regions and the spacer region have been modeled separately by the distance geometry and the homology method. The three parts are then connected to generate a three-dimensional structural model of kiwifruit metallothionein kiwi503. The result shows that there is no structural or energy barrier preventing either cysteine rich domain from independently forming a metal-sulfur chelating cluster. The method can also be applied to predicting the structures of the same type of other proteins.Kiwifruit metallothionein, kiwi503, is a typical plant metallothionein protein. It has 63 amino acid residues in two cysteine-rich regions and one spacer region of about 32 residues. In this note, the two cysteine-rich regions and the spacer region have been modeted separatety by the distance geometry and the homology method. The three parts are then connected to generate a three-dimensional structural modet of kiwifruit metallothionein kiwi503. The result shows that there is no structural or energy barrier preventing either cysteine rich domain from independently forming a metal-sulfur chetating cluster. The method can also be applied to predicting the structures of the same type of other proteins.

关 键 词：plant METALLOTHIONEIN HOMOLOGY DISTANCE GEOMETRY PROTEIN modeling. 

分 类 号：Q946[生物学—植物学]