Complete nucleotide sequence and genomic organization of Periplaneta fuliginosa densonucleosis virus  

作　　者：Haitao Guo Jiamin Zhang Yuanyang Hu 

机构地区：[1]Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China

出　　处：《Chinese Science Bulletin》2000年第19期1782-1786,共5页

摘　　要：We have cloned the replicative form of the Periplaneta fuliginosa densonucleosis virus (PfDNV) genome and determined its complete sequence. The sequence has 5 454 nucleotides (nt), the genome consists of an internal unique sequence flanked by inverted terminal repeats (201 nt). The first 122 nt at the 5’end and the terminal 122 nt at the 3’end of both plus and minus strands can fold into a typical hairpin structure. The genome contains seven major open reading frames (ORFs). The plus strand has 4 ORFs occupying the 5’half of the plus strand, whereas the others span the 5’ half of the minus strand. Two potential promoters were found at map units (m.u.) 3 and 97. Computer analysis of sequence homologies with other parvoviruses suggests that the plus strand of Pf DNV encodes very likely the nonstructural proteins and the minus strand probably encodes the structural proteins.We have cloned the replicative form of thePeriplaneta fuliginosa densonucleosis virus (PfDNV) genome and determined its complete sequence. The sequence has 5 454 nucleotides (nt), the genome consists of an internal unique sequence flanked by inverted terminal repeats (201 nt). The first 122 nt at the 5′ end and the terminal 122 nt at the 3′ end of both plus and minus strands can fold into a typical hairpin structure. The genome contains seven major open reading frames (ORFs). The plus strand has 4 ORFs occupying the 5′ half of the plus strand, whereas the others span the 5′ half of the minus strand. Two potential promoters were found at map units (m.u.) 3 and 97. Computer analysis of sequence homologies with other parvoviruses suggests that the plus strand ofPf DNV encodes very likely the nonstructural proteins and the minus strand probably encodes the structural proteins.

关 键 词：Perlplaneta fuliginosa DENSOVIRUS NUCLEOTIDE SEQUENCE GENOMIC organization. 

分 类 号：Q933[生物学—微生物学]