Cloning and sequence characteristics of the genomic gene of a rice metallothionein  

Cloning and sequence characteristics of the genomic gene of a rice motallothionein

作　　者：YU Lihua, LIU Jinyuan, Masaaki Umeda, Hirofumi Uchimiya & ZHAO Nanming Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Beijing 100084, China Institute of Molecular and Cellular Bioscie 

出　　处：《Chinese Science Bulletin》2000年第2期153-156,共4页

摘　　要：Northern blot analysis showed that a metallothionein gene, ricMT is expressed strongly in the stem of rice with an expression level that could be more than 100-fold stronger than in leaf blades. The results suggest that the 5’ upstream region flanking the coding sequence of the ricMT may contain a fairly strong promoter To elucidatr its regulation and promoter structure, the genomic clones of ricMT were screened out from a rice genomic library and a fragment of about 4084 bp was sequenced. The fragment included a 5’ upstream region of ca. 2970 bp, a transcription region of ca. 690 bp and a 3’ downstream region of ca. 420 bp. Computer analysis of the sequence homology showed that the 5’ upstream region included a putative TATA box, a putative CAAT box, and a typical metal-responsive element TGCGCGCG. The results will promote further understanding of the mechanisms of gene regulation and metal response of plant metallothionein proteins.Northern blot analysis showed that a metallothionein gene, ricMT is expressed strongly in the stem of rice with an expression level that could be more than 100-fold stronger than in leaf blades. The results suggest that the 5' upstream region flanking the coding sequence of the ricMT may contain a fairly strong promoter To elucidatr its regulation and promoter structure, the genomic clones of ricMT were screened out from a rice genomic library and a fragment of about 4084 bp was sequenced. The fragment included a 5' upstream region of ca. 2970 bp, a transcription region of ca. 690 bp and a 3' downstream region of ca. 420 bp. Computer analysis of the sequence homology showed that the 5' upstream region included a putative TATA box, a putative CAAT box, and a typical metal-responsive element TGCGCGCG. The results will promote further understanding of the mechanisms of gene regulation and metal response of plant metallothionein proteins.

关 键 词：RICE METALLOTHIONEIN GENOMIC gene regulatory sequence. 

分 类 号：Q785[生物学—分子生物学]