Cloning and expression of a cDNA encoding ribosomal protein S4 from Rice (Oryza sativa)  

作　　者：QU Lijia LI Donghui ZHANG Yi LIU Meihua GU Hongya CHEN Zhangliang 

机构地区：[1]National Laboratory of Protein Engineering and Plant Genetic Engineering,Peking University,Beijing 100871,China [2]De Montfort University,The Gateway,Leicester LE1 9BH,UK

出　　处：《Chinese Science Bulletin》2000年第2期168-173,共6页

摘　　要：A cDNA clone, pS4, has been isolated from a cDNA library prepared from rice anthers of about 1.0 mm in length. DNA sequence analysis and database search show that the cDNA encodes a protein which is highly homologous to eukaryotic 80S ribosomal protein subunit 4 (S4). Northern hybridization indicates that this gene expresses in all tissues analyzed although the expression level varies and it cannot be induced by mechanical wounding in leaves. Southern blot analysis demonstrates that this rice S4 gene is from a multigeneA cDNA clone, pS4, has been isolated from a cDNA library prepared from rice anthers of about 1.0 mm in length. DNA sequence analysis and database search show that the cDNA encodes a protein which is highly homologous to eukaryotic 80s ribosomal protein subunit 4 (S4). Northern hybridization indicates that this gene expresses in all tissues analyzed although the expression level varies and it cannot be induced by mechanical wounding in leaves. Southern blot analysis demonstrates that this riceS4 gene is from a multigene family.

关 键 词：RIBOSOMAL protein S4 cDNA CLONE monocotyledonous plant tissue specificity(rice) 1) The NUCLEOTIDE SEQUENCE data reported is in the EMBL NUCLEOTIDE SEQUENCE Database under the ACCESSION number Y15009. 

分 类 号：Q785[生物学—分子生物学] Q786