Construction of chimeric inducible promoters by elicitors of rice fungal blast pathogen and their expression in transgenic rice  

作　　者：LU Huafei MING Xiaotian QU Lijia LIU Meihua LI Jing GU Hongya CHEN Zhangliang 

机构地区：[1]State Key Laboratory of Protein Engineering and Plant Genetic Engineering,Peking University,Beijing 100871,China [2]Demontfort University,The Gateways,Leicester LEI 9BH,UK

出　　处：《Chinese Science Bulletin》2000年第3期242-246,共5页

摘　　要：The promoter fragments of wheat GstA1 and potato Gst1 have been amplified by PCR, cloned and fused respectively to the minimal promoter sequence of rice actin gene (Act1)) and its 5’ untranslated leader sequence together with GUS. The constructs with 2 chimeric promoters (WGA and PGA) have been transferred into rice in order to analyze their inducibility patterns in transgenic rice plants. The results show that: WGA and PGA are both inducible by elicitors of Pyricularia oryzae in transgenic rice cells; the intron I of rice Act1 gene is important for the heterogenic expression of monocot and dicot promoter elements in rice; and the Act1 minimal promoter and its 5’untranslated leader sequence produced low level background expression in rice.The promoter fragments of wheatGstA1 and potatoGst1 have been amplified by PCR, cloned and fused respectively to the minimal promoter sequence of rice actin gene (Act1)) and its 5′ untranslated leader sequence together withGUS. The constructs with 2 chimeric promoters (WGA and PGA) have been transferred into rice in order to analyze their inducibility patterns in transgenic rice plants. The results show that: WGA and PGA are both inducible by elicitors ofPyricularia oryzae in transgenic rice cells; the intron I of riceAct1 gene is important for the heterogenic expression of monocot and dicot promoter elements in rice; and theAct1 minimal promoter and its 5′ untranslated leader sequence produced low level background expression in rice.

关 键 词：pathogen-inducible CHIMERIC promoter gene EXPRESSION and regulation transgenic RICE RICE FUNGAL BLAST disease. 

分 类 号：S511[农业科学—作物学]