The conformation alteration of mouse hepatic histones after reacting with nicotine in vitro  

作　　者：Xiaohong Wu Hongfang Sun Yuanfang Liu 

机构地区：[1]Peking Univ, Dept Tech Phys, Beijing 100871, Peoples R China

出　　处：《Chinese Science Bulletin》2000年第9期825-830,共6页

摘　　要：UV differential spectroscopy, fluorescence spectroscopy and circular dichroism (CD) spectroscopy assays have been applied to studying the conformation alteration of mouse hepatic histones H1 and H3 after reacting with nicotine in vitro. The results indicate that their conformation changes from regular form to random form with the increasing reaction dose of nicotine. The adduction of nicotine or its metabolites with histones H1 and H3 accounts for the conformation alteration. Nicotine may affect the structure, function and expression of genes of chromosome by changing the conformation of histones.UV differential spectroscopy, fluorescence spectroscopy and circular dichroism (CD) spectroscopy assays have been applied to studying the conformation alteration of mouse hepatic histones H1 and H3 after reacting with nicotinein vitro. The results indicate that their conformation changes from regular form to random form with the increasing reaction dose of nicotine. The adduction of nicotine or its metabolites with histones H1 and H3 accounts for the conformation alteration. Nicotine may affect the structure, function and expression of genes of chromosome by changing the conformation of histones.

关 键 词：NICOTINE HISTONE CONFORMATION genotoxicity. 

分 类 号：Q51[生物学—生物化学]