Using laser confocal scanning microscope to study ischemia-hypoxia injury in rat brain slice  

作　　者：WANG Xiaoying XING Hong HE Qihua XU Jialing WU Benjie 

机构地区：[1]Department of Biophysics,Beijing Medical University,Beijing 100083,China [2]Analytic Center of Medico-Pharmacy,Beijing Medical University,Beijing 100083,China

出　　处：《Chinese Science Bulletin》2000年第1期49-51,共3页

摘　　要：The level of lipid peroxidation and cellular necrosis in rat living brain slices during brain ischemia-hypoxia injury have been observed using a laser confocal scanning microscope (LCSM) with double labeling of fluorescent probes D-399 (2, 7-dichlorofluorescin diacetate) and propidium iodide (Pl). The hypoxia and/or reoxygenation injury in rat brain slices is markedly decreased by pretreatment with L-NG-nitro-arginine (L-NNA) and N-acetylcysteine (NAC), showing that the nitric oxide (NO) and other free radicals play an important role in brain ischemia-hypoxia injury.The level of lipid peroxidation and cellular necrosis in rat living brain slices during brain ischemia-hypoxia injury have been observed using a laser confocal scanning microscope (LCSM) with double labeling of fluorescent probes D-399 (2, 7-dichlorofluorescin diacetate) and propidium iodide (PI). The hypoxia and/or reoxygenation injury in rat brain slices is markedly decreased by pretreatment with L-NG-nitro-arginine (L-NNA) and N-acetylcysteine (NAC), showing that the nitric oxide (NO) and other free radicals play an important role in brain ischemia-hypoxia injury.

关 键 词：LASER confocal scanning MICROSCOPE brain ischemia-hypoxia INJURY lipid peroxide 2 7-dichIorofluorescin diacetate. 

分 类 号：R363[医药卫生—病理学]