Swelling-activated chloride currents in embryonic chick heart cells  

鸡胚心肌细胞的膨胀激活氯电流<英文>

作　　者：魏华[1] 梅岩艾[1] 吴明明[1] 孙家庭[1] 周汉清[1] 张志鸿[1] 

机构地区：[1]复旦大学生理学和生物物理学系立人实验室

出　　处：《Acta Pharmacologica Sinica》2000年第11期28-32,共5页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Fotmdation of China (No 39730150).

摘　　要：AIM: To characterize a swelling-activated chloride cur-rent, I(Cl,swell) in white Leghorn chick heart cells and the effects of chlorpromazine (CPZ) effects. METH-ODS: The patch-clamp technique in the whole-cell con-figuration was used. RESULTS: Hyposmotic swelling elicited I(Cl,swell) in white Leghorn chick heart cells. The current amplitude increased from (452±200) pA to (849±373) pA with a reduction of osmolarity from 300 mmol·L-1 to 270 mmol·L-1. 4', 4-Diisothio-cyanostilbene-2,2'-disulphonic acid (DIDS) 100 μmol·L-1 decreased I(Cl, swell) from (1196 ±505) pA to (830±328) pA in hyposmotic solution. In white Leghorn chick heart cells I(Cl, swell) was not induced by CPZ 30 μmol·L-1, which is different from the case of E coli spheroplast. CONCLUSION: Swelling-activated chlo-ride current was activated by hyposmotic swelling in white Leghorn chick heart cells. The mechanism for activating the current is different from that of mechanosensitive ion channels of E coli.目的:研究白羽鸡胚心肌细胞的膨胀激活氯电流及氯丙嗪对其的模拟作用.方法:膜片箝技术的全细胞记录模式.结果:低渗膨胀在白羽鸡胚心肌上诱发出一个可逆变化的氯电流,渗透压从300 mmol·L^(-1)减小至270 mmol·L^(-1)时,该电流从(452±200)pA增加到(849±373)pA.DIDS 100 μmol·L^(-1)使氯电流从(1196±505)pA减至(830±328)pA.I(Cl,swell)不能被CPZ 30μmol·L^(-1)模拟出来,该结果不同于大肠杆菌原生质体的结果.结论:白羽鸡胚心肌细胞的膨胀激活氯通道能被低渗激活,其激活的机制与大肠杆菌的机制敏感性离子通道激活的机制不同.

关 键 词：swelling activation chick embryo MYOCARDIUM chloride channels patch-clamp techniques CHLORPROMAZINE 

分 类 号：R96[医药卫生—药理学]