Dimethylamiloride-induced differentiation of HL-60 cells  

二甲基氨氯吡咪诱导HL-60细胞分化(英文)

作　　者：黎运源[1] 梁念慈[1] 江丽 吴铁 林小粤 

机构地区：[1]广东医学院医用生化研究所 [2]广东天然药物研究开发重点实验室

出　　处：《Acta Pharmacologica Sinica》2000年第5期63-67,共5页中国药理学报（英文版）

基　　金：Project support by Guangdong Research Foundation for Key Field.№ 9306.

摘　　要：AIM: To study the effect of 5-( N, N-dimethyl) amiloride (DMA) on the proliferation and differentiation of HL-60 cells in vitro. METHODS: MTT assay to test cytotoxicity; cell staining and NBT reduction to test cell differentiation. RESULTS: DMA inhibited HL-60 cells growth in a concentration-dependent manner, and IC50 value for 96 h was 31.7 (95 % confidence limits: 6.3 - 57.1) μmol·L-1. DMA also induced granulocy t-ic differentiation in HL-60 cells. The percentage of differentiating cells increased from 6.5 % to 70 % after DMA 100μmol·L-1 treatment for 3 d. The differentiating effect of DMA was distinguishable from amiloride, 5-(N-ethyl-N-isopropyl) amiloride (EIPA), and (5-(N-methyl-N-isobutyl)amiloride (MIA). None among the amiloride, EIPA, and MIA were capable of triggering the differentiation of HL-60 cells. CONCLUSION: DMA inhibited the proliferation of HL-60 cells and induced differentiation of HL-60 cells.目的:研究阿米洛利衍生物二甲基阿米洛利(DMA)对培养的HL-60细胞增殖和分化的影响。方法:MTT法测定细胞增殖,细胞染色和NBT还原试验测定细胞分化。结果:DMA以浓度依赖方式抑制HL-60细胞增殖,药物作用96小时测得半数抑制浓度(IC_(50))为31.7(95％可信限为6.3-57.1)μmol·L^(-1)。DMA诱导HL-60细胞向粒细胞系分化。加入DMA100μmol·L^(-1)作用3天,细胞分化率从6.5％升至70％。阿米洛利及阿米洛利的另二个衍生物对细胞分化无明显作用。结论:二甲基阿米洛利抑制HL-60细胞增殖和诱导HL-60细胞分化。

关 键 词：cell division cell differentiation HL-60 cells AMILORIDE inhibitory concentration 50 

分 类 号：R73-3[医药卫生—肿瘤]