Tetrahydroprotoberberines inhibit lipid peroxidation and scavenge hydroxyl free radicals  

四氢原小檗碱同类物抑制脂质过氧化和清除羟自由基(英文)

作　　者：金昔陆[1] 邵悦[2] 王铭洁[1] 陈丽娟[2] 金国章[2] 

机构地区：[1]上海医科大学药学院药理教研室 [2]中国科学院上海药物研究所

出　　处：《Acta Pharmacologica Sinica》2000年第5期95-98,共4页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China, № 39870898.

摘　　要：AIM: To study the effects of tetrahydroprotoberberines (THPB) on rat liver and brain lipid peroxidation (LPO) and oxygen free radicals generation. METHODS: The malondialdehyde (MDA) levels in rat brain and liver ho-mogenates, induction of MDA by Fe2+-Vit C in mitochondria, OH generation by Fenton reaction, and O2 generation by pyrogallol oxidation were observed in vitro. RESULTS: (1) THPB lowered the MDA contents in the liver homogenate and mitochondria, and the IC50 values of l-THPB-18 and l-stepholidine (SPD) in the liver mitochondria were 3.1 and 12.7 μmol·L-1 respectively. SPD decreased the MDA contents in the brain homogenate and mitochondria with IC50 values of 102 and 35.0μmol·L-1 respectively. (2) THPB scavenged OH, and the IC50 values of l-THPB-18 and SPD were 0.21 and 3.8μmol·L-1 respectively, but no effect on O2 was observed. CONCLUSION: THPB could reduce the MDA contents and scavenge OH and THPB-18 was the most potent amongst them.目的:研究THPB对大鼠肝、脑脂质过氧化(LPO)和氧自由基的影响。方法:体外测定大鼠肝、脑匀浆LPO和Fe^(2+)-Vit C诱导肝、脑线粒体LPO产生的MDA,Fenton反应产生OH和邻苯三酚氧化产生O_2^-。结果:(1)THPB减少肝匀浆和线粒体MDA,其中l-THPB-18和SPD的IC_(50)为3.1和12.7μmol·L^(-1)。SPD减少脑匀浆和脑线粒体MDA,IC_(50)分别为102和35.0μmol·L^(-1)。(2)THPB能清除OH^-,其中I-THPB-18和SPD的IC_(50)为0.21和3.8μmol·L^(-1);但对O_2无抑制作用。结论:THPB能减少MDA和清除OH^-,其中THPB-18作用最强。

关 键 词：STEPHOLIDINE lipid peroxidation MALONDIALDEHYDE MITOCHONDRIA free radicals 

分 类 号：R96[医药卫生—药理学]