Antisense oligodeoxynucleotide inhibits vascular endothelial growth factor in human glioma cells^1  

反义寡脱氧核苷酸抑制人胶质瘤细胞的血管内皮生长因子(英文)

作　　者：郑世曦[1] 周利军[1] 朱兴族 金由辛[2] 

机构地区：[1]中国科学院上海药物研究所药理室 [2]中国科学院上海生物化学研究所分子生物学国家重点实验室

出　　处：《Acta Pharmacologica Sinica》2000年第3期21-24,共4页中国药理学报（英文版）

基　　金：Project supported by The High-Tech Research and Development Plan (No 102-08-8);National New Drug Research foundation (No 96-901-05-87).

摘　　要：AIM: To study the effects of antisense vascular endothe-lial growth factor (VEGF) oligodeoxynucleotide (ODN) on the expression of VEGF in human glioma cell line (A172 cells). METHODS: VEGF mRNA level was measured by semiquantification reverse transcriptase poly-merase chain reaction (RTPCR). VEGF protein expression in the cells was determined by immunohistoche-mistry. VEGF protein level in the media was measured by ELISA. RESULTS: When the cells were treated with antisense VEGF ODN (6.25-50μmol·L-1), VEGF mRNA level in the cells decreased remarkably in a concentration-dependent manner. No change was found when the cells were treated with sense or missense ODN. When the cells were treated with antisense VEGF ODN 25μmol·L-1, VEGF protein level decreased greatly both in the cells and the media. CONCLUSIONS: Antisense VEGF ODN inhibited VEGF expression specifically in A172 cells in vitro and thus the results provided the basis for the further studies in vivo.目的:了解反义VEGF寡脱氧核苷酸(ODN)对人胶质细胞瘤系(A172细胞)VEGF表达的抑制作用。方法:应用半定量PCR、免疫组化方法分别了解细胞内VEGF mRNA和蛋白的变化,并利用ELISA检测培养上清液中VEGF蛋白的含量。结果:A172细胞经反义VEGF ODN作用后VEGF mRNA的表达量明显减少,且随其浓度的增加而明显减少,但其表达量不受正义和错义VEGF ODN的影响。当A172细胞经50μmol·L^(-1)反义VEGF ODN作用后,细胞内及上清液内VEGF蛋白水平较对照显著减少。结论:反义VEGF ODN特异地抑制A172细胞VEGF mRNA和蛋白的表达。

关 键 词：antisense oligodeoxynucleotides GLIOMA endothelial growth factor reverse transcriptase polymerase chain reaction immunohistochemistry enzyme-linked immunosorbent assay cultured tumor cells messenger RNA 

分 类 号：R73-3[医药卫生—肿瘤]