Screening and identification of mimotopes of LPS conservative epitope from random phage display peptide library  

作　　者：文维延 韩强涛 富宁 

出　　处：《Journal of Medical Colleges of PLA(China)》2000年第4期262-265,共4页中国人民解放军军医大学学报（英文版）

基　　金：Supported by Natural Science Foundation of Guangdong Province, No. 970334

摘　　要：Objective: To screen and identify the mimotopes of lipopolysaccharide (LPS) epitope. Methods: A random phage display dodecapeptide library was screened with monoclonal antibody 2B4 specifically against LPS conservative epitope. The positive clones were identified by phage EUSA and competitive inhibition assay with either S. typhimurium T861 LPS or E. coli Olll:B4 LPS. Results: After 3 rounds of biopanning, the clones bound with monoclonal antibody 2B4 were well enriched with the positive rate of 80%. The bindings between 12 positive phage clones and screening antibody were inhibited by both kinds of LPS. The positive peptide sequences were deduced from the corresponding DNA sequences. There were some identical sequences among them: PPQWFFSQPQL (5/12, 41. 7%), LPQYFW NTATTA (3/12, 25%), FPQNHWNVPWAT(2/12, 16. 6% ),HSQSFWNAPLAM and AHPWTHGYFPPL (l/12, 8. 3% ). Conclusion: The peptides screened with 2B4 antibody are mimotopes of LPS conservative epitope.Objective: To screen and identify the mimotopes of lipopolysaccharide (LPS) epitope. Methods: A random phage display dodecapeptide library was screened with monoclonal antibody 2B4 specifically against LPS conservative epitope. The positive clones were identified by phage EUSA and competitive inhibition assay with either S. typhimurium T861 LPS or E. coli Olll:B4 LPS. Results: After 3 rounds of biopanning, the clones bound with monoclonal antibody 2B4 were well enriched with the positive rate of 80%. The bindings between 12 positive phage clones and screening antibody were inhibited by both kinds of LPS. The positive peptide sequences were deduced from the corresponding DNA sequences. There were some identical sequences among them: PPQWFFSQPQL (5/12, 41. 7%), LPQYFW NTATTA (3/12, 25%), FPQNHWNVPWAT(2/12, 16. 6% ),HSQSFWNAPLAM and AHPWTHGYFPPL (l/12, 8. 3% ). Conclusion: The peptides screened with 2B4 antibody are mimotopes of LPS conservative epitope.

关 键 词：PHAGE peptide library MIMOTOPE LIPOPOLYSACCHARIDE 

分 类 号：R341[医药卫生—基础医学]