缺血预处理对肝脏抗氧化能力的影响及机制  

The Effect and Mechanism of Ischemic Preconditioning on Hepatic Antioxidant Ability

作　　者：王野[1] 葛云洁[1] 赵宜仁[1] 

机构地区：[1]青岛市市立医院,266011

出　　处：《青岛医药卫生》2000年第5期321-323,共3页Qingdao Medical Journal

摘　　要：目的 在大鼠肝脏原位缺血再灌注(I/R)模型上观察缺血预处理(IP)对肝脏抗氧化能力的影响及其机制。方法 在大鼠肝脏原位缺血再灌注模型上,心包穿刺抽血,切取肝组织,分别进行血清ALT、AST检测,肝组织MDA、SOD、Cat、GSH-PX测定。结果 发现预处理组与缺血再灌注组相比血清中ALT、AST水平及肝组织匀浆中MDA的含量降低,而肝组织中SOD、Cat及GSH-PX的活性均不同程度增高,多数检测指标各预处理组之间均无明显差异。结论 实验表明缺血预处理能增强缺血再灌注肝脏的抗氧化能力,增加预处理的次数并不能增加预处理的效果。腺苷在缺血预处理过程中起着重要作用。Objective This study investigated the effect and mechanism of ischemic preconditioning (IP)on the model of rat liver subjected to the in situ schemia/reperfusion(I/P)injury. Methods To meosure the concentrations of ALT, AST and hepatic SOD,Cat,GSH-PX activity on the rat model. Results The results revealed the levels of serum ALT, AST and the contents of MDA in IP groups were lower than those in I/R group(P<0. 05),while the activity of SOD,Cat,GSH-PX in liver tissues were more or less increased. Most observations had no significant changes(PX). 05)when IP groups were compared with each other. Conclusion It was showed that IP could raise the antioxidant ability of liver subjected to I/R,but increasing the times could not improve the effect of IP. Adenosine played an important role in the course of IP.

关 键 词：肝脏 缺血预处理 缺血再灌注 抗氧化能力 

分 类 号：R363[医药卫生—病理学]