Crystallization and preliminary crystallographic studies of insulin T_3R_3 states created in different conditions  

作　　者：AI Xi ZENG Zonghao HUANG Peitang Jan Markussen WANG Dacheng 

机构地区：[1]Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China [2]Institute of Biotechnology,Academy of Military Medical Sciences,Beijing 100071,China [3]Novo Research Insti tute,Novo Alle,DK_2880 Bagsvaerd,Denmark

出　　处：《Chinese Science Bulletin》1998年第1期59-62,共4页

基　　金：theNationalHigh_TechProgramofChina

摘　　要：In the presence of a series of small organic molecules including 1, 4 dioxane, DMF, isopropanol, rDNA human insulin, mutants A21 Asn→Ser and (A21 Asn→Ser, B27 Thr→Arg) human insulin have been crystallized in both Zn 2+ containing and Zn 2+ free system. Preliminary crystallographic analyses show that they all belong to T 3R 3 structural type. The results indicate that, except phenolic derivatives and lyotropic anions reported before, some other appropriate organic molecules can also induce the T 3R 3 conformation transition of insulin.In the presence of a series of small organic molecules including 1, 4 dioxane, DMF, isopropanol, rDNA human insulin, mutants A21 Asn→Ser and (A21 Asn→Ser, B27 Thr→Arg) human insulin have been crystallized in both Zn 2+ containing and Zn 2+ free system. Preliminary crystallographic analyses show that they all belong to T 3R 3 structural type. The results indicate that, except phenolic derivatives and lyotropic anions reported before, some other appropriate organic molecules can also induce the T 3R 3 conformation transition of insulin.

关 键 词：INSULIN T_3R_3 structure EFFECTOR CRYSTALLIZATION preliminary crystallographic study cell constants. 

分 类 号：Q591.2[生物学—生物化学]