Protooncogene c-fos overexpression in NADPH-d positive neurons of the amygdala in soman induced seizures in the rat  

作　　者：景德强 陈尔瑜 余争平 程天民 

出　　处：《Journal of Medical Colleges of PLA(China)》1998年第1期43-48,共6页中国人民解放军军医大学学报（英文版）

摘　　要：Fos immunohistochemistry and NADPH-d histochemistry and double labeling of the 2 methods were used to study therelationship between c-Fos expressed neurons and NADPH-d positive neurons of the amygdala in soman-induced seizures in the rat.It was found that protooncogene c-fos was overexpressed in the amygdala from 1.5 h to 2 d after soman-induced seizures occurred.c-fos overexpression was in the persisting and delayed pattem. The distribution of Fos immunoreactively positive neurons was sub-nuclear specific. About 10% of the Fos-positive neurons were NADPH-d positive and almost all the NADPH-d positive neuronswere also stained with Fos immunohistochemistry. Our findings together with those reported in our previous paper in which c-fosexpression and NOS activation were found to share the common upstream of the intracellular signal transducing cascade mediatedthrough NMDA receptor depended Ca2+ influx suggest that c-fos expression and nitric oxide neurotoxicity mighy exist in the amygdala lesions after soman induced seizures occurred.Fos immunohistochemistry and NADPH-d histochemistry and double labeling of the 2 methods were used to study therelationship between c-Fos expressed neurons and NADPH-d positive neurons of the amygdala in soman-induced seizures in the rat.It was found that protooncogene c-fos was overexpressed in the amygdala from 1.5 h to 2 d after soman-induced seizures occurred.c-fos overexpression was in the persisting and delayed pattem. The distribution of Fos immunoreactively positive neurons was sub-nuclear specific. About 10% of the Fos-positive neurons were NADPH-d positive and almost all the NADPH-d positive neuronswere also stained with Fos immunohistochemistry. Our findings together with those reported in our previous paper in which c-fosexpression and NOS activation were found to share the common upstream of the intracellular signal transducing cascade mediatedthrough NMDA receptor depended Ca2+ influx suggest that c-fos expression and nitric oxide neurotoxicity mighy exist in the amygdala lesions after soman induced seizures occurred.

关 键 词：C-FOS NITRIC oxide AMYGDALA SOMAN seilzure 

分 类 号：R392.1[医药卫生—免疫学]