PCR及限制性酶切技术检测急性髓细胞白血病H-ras基因点突变  

Detection of H - ras Gene Point Mutation in Acute Myeloid Leukemia with PCR and Restriction Engzme Digestion Assay

作　　者：李栋梁[1] 宣宝和[1] 温进坤[2] 魏素珍[2] 

机构地区：[1]白求恩国际和平医院血液内科 [2]河北医科大学基础所

出　　处：《解放军医药杂志》1997年第2期91-93,共3页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

摘　　要：为了解急性髓细胞白血病(AML)H-ras基因点突变的情况,应用聚合酶链反应(PCR)和限制性酶切图谱分析技术,对55例初诊AML患者进行了检测,发现16例(29.1％)存在H-ras基因点突变,此突变与AML的发生及其某些临床指标有一定关系。H—ras基因突变可望成为AML诊断分型、指导治疗及判断预后的一项有价值的指标。To inquire into the situation of H - ras gene point mutation in acute myeloid Leukemia (AML)55 cases with AML at diagnosis were detected. The result showed that 16 cases (29.1% ) contained H - ras gene point mutation, and the mutation had certain correlation with Leukemogenesis and some clinical parametres of AML . H - ras gene mutation will be expected to become a Valuable Predictor in diagnosis typing, directing treatment and determining outcome of AML.

关 键 词：RAS基因 突变 聚合酶链反应 髓细胞性白血病 

分 类 号：R733.71[医药卫生—肿瘤]