海带雌性原生质体的分离与培养  

ISOLATION AND CULTURE OF FEMALE HAPLOID PROTOPLASTS FROM LAMINARIA JAPONICA

作　　者：秦松[1] 王希华[1] 童顺 曾呈奎[1] 

机构地区：[1]中国科学院海洋研究所,青岛266071

出　　处：《海洋与湖沼》1995年第S1期126-129,142,共5页Oceanologia Et Limnologia Sinica

基　　金：国家科委资助;山东省科委合同攻关项目

摘　　要：于1993年1月-1993年4月，为了制备遗传性纯一的基因工程受体，用酶法从海带雌配子体单性生殖系分离雌性单倍体原生质体。从皱纹盘鲍消化腺及消化器官提取出鲍酶，在250℃下、1％（W／V）的鲍酶与2％（W／V）的纤维素酶（OnozukaR－10）合用能有效解离单细胞和原生质体。海带雌性原生质体经2d培养再生了细胞壁。Female haploid protoplasts were isolated in January to April, 1993 from female gametophytes of Laminaria japonica using abalone enzymes extracted from the dige-stive organs of the abalone Haliotis discus hannai. Single cells and protoplasts could be isolated effectively from filamentous gametophytes after incubation with 1% (W/ V) abalone enzymes and 2%(W/V) cellulase (Onozyka R-10) at 25℃. Microscopy and fluorescent microscopy revealed that viable female haploid protoplasts were iso-lated, and that cell walls were regenerated after 2 days.

关 键 词：海带 雌配子体 原生质体 鲍酶 纤维素酶 

分 类 号：Q943[生物学—植物学]