细胞原位杂交法检测肿瘤细胞中C-myc基因的表达  

Detectation of C-myc gene expression in tumor cell by in situ hybridization

作　　者：李少英[1] 李月秋[1] 马洪星[1] 王景华[2] 陈志[2] 韩秀坤[2] 

机构地区：[1]佳木斯医学院生物教研室 [2]佳木斯医学院免疫教研室

出　　处：《黑龙江医药科学》1994年第4期6-7,共2页Heilongjiang Medicine and Pharmacy

摘　　要：本文采用生物素标记的C-myc基因作探针,通过细胞原位分子杂交技术,对人舌癌、膀胱癌和正常细胞中C-myc基因的转录量进行了定性和半定量分析。结果表明与正常细胞相比,舌癌和膀胱癌细胞中C-myc基因异常高度表达。The qualitive and semiquantitve analysts was made of the transcripts of C-myc gene in cells from tongue and bladder cancer using biotin-labeled C-myc gene as probe by in situ hybridization. The results indicate that the transcripts of C-myc gene in tongue cancer and bladder cancer cells are higher than those in normal cell.

关 键 词：C—myc基因表达 转录 细胞原位分子杂交技术 

分 类 号：R730.2[医药卫生—肿瘤]