COEXISTENCE OF FOS-LIKE PROTEIN IN TYROSINE HYDROXYLASE-LIKE IMMUNOREACTIVE NEURONS IN THE HINDBRAIN OF ARTS FOLLOWING PERIPHERAL ELECTRICAL STIMULATION  

作　　者：Ji Rurong Fang Yuan Zhang Qin Han Jisheng, Department of Physiology, Beijing Medical University, Beijing 100083, China 

出　　处：《Chinese Journal of Biomedical Engineering(English Edition)》1993年第1期39-45,共7页中国生物医学工程学报（英文版）

基　　金：Chinese postdoctoral foundation;a grand from the National Institute of Drug Abuse, USA (DA 03983)

摘　　要：In the present study we have found that proto-oncogene c-fos protein can expressin the noradrenergic neurons of rat hindbrain following peripheral electrical stimulation. Ratswere given peripheral electrical stimulation via thin stainless steel pins inserted into the pointsnear knee joint (S36) and ankle joint (Sp6) which mimic the manipulation of electroacupuncture(EA) performed in humans. Animals were perfused for double staining immunohistochemistry 2hafter the termination of EA. In rats subjected to EA stimulation Fos-like protein was found in thetyrosine hydroxylase (TH)-like immunoreactive neurons in rat hindbrain. The Fos and TH coex-isting neurons were distributed in the locus coeruleus, solitary tract nucleus, ventrolateral medul-la, periaqeductal gray, as well as superior colliculus. The percentage of the coexisting neuronscompared with the total number of neurons containing Fos-like protein in these nuclei rangedfrom 6% to 32%. The results suggest that the noradrenergic neurons in these regions may be ac-tivted by acupuncture stimulation.In the present study we have found that proto-oncogene c-fos protein can express in the noradrenergic neurons of rat hindbrain following peripheral electrical stimulation. Rats were given peripheral electrical stimulation via thin stainless steel pins inserted into the points near knee joint (S36) and ankle joint (Sp6) which mimic the manipulation of electroacupuncture (EA) performed in humans. Animals were perfused for double staining immunohistochemistry 2h after the termination of EA. In rats subjected to EA stimulation Fos-like protein was found in the tyrosine hydroxylase (TH)-like immunoreactive neurons in rat hindbrain. The Fos and TH coex- isting neurons were distributed in the locus coeruleus, solitary tract nucleus, ventrolateral medul- la, periaqeductal gray, as well as superior colliculus. The percentage of the coexisting neurons compared with the total number of neurons containing Fos-like protein in these nuclei ranged from 6% to 32%. The results suggest that the noradrenergic neurons in these regions may be ac- tivted by acupuncture stimulation.

关 键 词：COEXISTENCE Fos-like protein TYROSINE HYDROXYLASE PERIPHERAL electrical stimu-lation electroacupuncture HINDBRAIN 

分 类 号：R318[医药卫生—生物医学工程]