Binding of HMG Proteins to the 5'_Flanking Sequence of Human β-Globin Gene  

作　　者：钱若兰 陈雅娣 宋秋宝 胡玉龙 

机构地区：[1]Shanghai Institute of Cell Biology, Academia Sinica, Shanghai 200031, PRC

出　　处：《Science China Chemistry》1993年第1期81-88,共8页中国科学（化学英文版）

基　　金：Project supported by grants from Academia Sinica.

摘　　要：Our previous studies have identified that there are at least three regulatory regions (two negative regions and one positive region) in the 5'-flanking sequence of human β-globin gene (-610 to +1 bp). The binding of HMG proteins to both negative regulatory regions was examined by the gel mobility shift and DNase I protection assays.In gel mobility shift assay,we observed that HMG proteins 1 and 2 could bind to both negative regulatory regions (NCR1 and NCR2).Using the gel shift competition assay,we identified that the binding proteins between the two regions are different from each other.DNase I protection analysis shows that HMG proteins 1 and 2 only bind to one site (between-560 and-533 bp) in NCR1.However,two protected regions can be detected in NCR2, one between-272 and-252 bp relative to the cap site, the other between-306 and-329 bp.We also observed that HMG proteins 14 and 17 could not bind to both negative regions, so it seems that HMG proteins 1 and 2 may play an important role in the regulation of β-globin expression through DNA-protein interaction or through protein-protein interaction.Our previous studies have identified that there are at least three regulatory regions (two negative regions and one positive region) in the 5’-flanking sequence of human β-globin gene (-610 to +1 bp). The binding of HMG proteins to both negative regulatory regions was examined by the gel mobility shift and DNase I protection assays.In gel mobility shift assay,we observed that HMG proteins 1 and 2 could bind to both negative regulatory regions (NCR1 and NCR2).Using the gel shift competition assay,we identified that the binding proteins between the two regions are different from each other.DNase I protection analysis shows that HMG proteins 1 and 2 only bind to one site (between-560 and-533 bp) in NCR1.However,two protected regions can be detected in NCR2, one between-272 and-252 bp relative to the cap site, the other between-306 and-329 bp.We also observed that HMG proteins 14 and 17 could not bind to both negative regions, so it seems that HMG proteins 1 and 2 may play an important role in the

关 键 词：cis-acting REGULATORY sequences HMG PROTEINS Β-GLOBIN gene. 

分 类 号：O6[理学—化学]