The Production of Monoclonal Antibody to Androstenedione and the Effect of Passive Immunization on Xinjiang Fine-Wool Sheep With It  

作　　者：吕兆启 马纪萱 周常文 王云芳 王军 向际尧 高远 曾宪黎 

机构地区：[1]Xinjiang Institute of Chemistry, Academia Siniea, Urumqi 830011, PRC

出　　处：《Science China Chemistry》1993年第6期693-701,共9页中国科学（化学英文版）

基　　金：Project supported by one of the National Research Subjects in the Seventh Five-Year Science and Technology Plan and the National Natural Science Foundation of China

摘　　要：Five hybridomas stably secreting monoclonal antibodies (McAbs) to androstenedione were prepared by using artificially synthesized androstenedione-11α-succinyl conjugate with bovine serum albumin (BSA) as antigen. These McAbs showed slightly high cross-reactivity with testosterone (8.1—12.3%) and estrone (0.8—2.5%) and high affinities ranging from 2.0×10~7 to 2.8×10~8 L/M. They were all of the IgG_1 subclass. Xinjiang finewool ewes which were passively immunized with McAbs had higher circulating levels of progesterone (P) and luteinizing hormone (LH) than those of control ewes in two oestrous cycles and these changes led to increased ovulation rate and twin lambs born in young ewes.Five hybridomas stably secreting monoclonal antibodies （McAbs） to androstenedione were prepared by using artificially synthesized androstenedione-11α-succinyl conjugate with bovine serum albumin （BSA） as antigen. These McAbs showed slightly high cross-reactivity with testosterone （8.1—12.3%） and estrone （0.8—2.5%） and high affinities ranging from 2.0×10⁷ to 2.8×10⁸ L/M. They were all of the IgG₁ subclass. Xinjiang finewool ewes which were passively immunized with McAbs had higher circulating levels of progesterone （P） and luteinizing hormone （LH） than those of control ewes in two oestrous cycles and these changes led to increased ovulation rate and twin lambs born in young ewes.

关 键 词：ANDROSTENEDIONE MONOCLONAL antibody Xinjiang fine-wool sheep TWIN ovulations TWIN LAMBS born. 

分 类 号：O6[理学—化学]