Modified reversed-phase high performance liquid chromatography for the determination of leukotriene B_4 and 5-hydroxyeicosatetraenoic acid in peripheral neutrophils of rabbits  

作　　者：张敏 尤忠义 雷政宏 王风君 

机构地区：[1]Burn Research Institute,Southwest Hospital,Third Military Medical College,Chongqing 630038

出　　处：《Journal of Medical Colleges of PLA(China)》1993年第2期192-196,共5页中国人民解放军军医大学学报（英文版）

摘　　要：Modification was made on the reversed-phase high performance liquid chromatography(RP-HPLC)with Yue et al's method as a base.The modified RP-HPLC was used to detect leukotriene B_4(LTB_4)and 5-hydroxyeicosatetraenoic acid(5-HETE).It was found that the modified method has the merits of simpler procedures,shorter testing time and more satisfactory efficacy.Modification was made on the reversed-phase high performance liquidchromatography（RP-HPLC）with Yue et al’s method as a base.The modified RP-HPLCwas used to detect leukotriene B₄（LTB₄）and 5-hydroxyeicosatetraenoic acid（5-HETE）.Itwas found that the modified method has the merits of simpler procedures,shortertesting time and more satisfactory efficacy.

关 键 词：leukotriene B_4 5-hydroxyeicosatetraenoic acid chromatography high performance liquid NEUTROPHILS animal RABBITS 

分 类 号：R[医药卫生]