EFFECTSOFVASOACTIVEINTESTINALPEPTIDEONACTIVITIESOFSUCCINICDEHYDROGENASEANDALKALINEPHOSPHATASEINRATHEPATOMACELLS  

作　　者：Huang Xiaofeng Zhang Yuanqiang Bu Yongan Su Huici (Department of Histology and Embryology,Fourth Military Medical University,Xi’an 710032) 

出　　处：《中国组织化学与细胞化学杂志》1993年第4期350-354,共5页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：Using cytochemical method,microspectrophotometry and image analysis,effects of va-soactive intestinal peptide(VIP)on activities of succinic dehydrogenase(SDH)and alkalinephosphatase(ALP)in rat hepatoma cells were studied in vitro.The results showed that thehepatoma cell expressed potent positive reactions of SDH and ALP,the positive positionswere located at the cell membranes and/or cytoplasm.Having been treated with VIP,ALPdecreased obviously in activity(P【0. 01,compared with hepatoma cells untreated by VIP).The sites of ALP activty were chiefly located at the cell membranes,particularly at the cell-cell contacts.Cultured rat hepatoma cells had intensive SDH activity in their cytoplasm.Compared with untreated eclls,there was no marked difference in the intensity of SDH activ-ity in VIP-treated hepatoma cells(P】0.05).Using cytochemical method,microspectrophotometry and image analysis,effects of va- soactive intestinal peptide(VIP)on activities of succinic dehydrogenase(SDH)and alkaline phosphatase(ALP)in rat hepatoma cells were studied in vitro.The results showed that the hepatoma cell expressed potent positive reactions of SDH and ALP,the positive positions were located at the cell membranes and/or cytoplasm.Having been treated with VIP,ALP decreased obviously in activity(P<0. 01,compared with hepatoma cells untreated by VIP). The sites of ALP activty were chiefly located at the cell membranes,particularly at the cell- cell contacts.Cultured rat hepatoma cells had intensive SDH activity in their cytoplasm. Compared with untreated eclls,there was no marked difference in the intensity of SDH activ- ity in VIP-treated hepatoma cells(P>0.05).

关 键 词：Vasoactive INTESTINAL PEPTIDE ALKALINE phophatase Succinic deydrogenase HISTOCYTOCHEMISTRY Imaging processing Computer-assisted TUMOR cell culture 

分 类 号：Q[生物学]