马钱子碱抗血小板聚集及其对凝血酶诱导血小板内游离钙的变化  

THE PLATELET AGGREGATION INHIBITED BY BRUCINE AND MEASUREMENT OF INTRACELLULAR FREE Ca^(2+) CONCENTRATION IN HUMAN PLATELET URING FURA-2/AM

作　　者：朱燕娜[1] 傅润芳 岳京丽[2] 

机构地区：[1]河南医科大学药理学教研室,郑州450052 [2]河南医科大学附属卫生学校药理学教研室

出　　处：《河南医学研究》1993年第3期217-220,共4页Henan Medical Research

摘　　要：①以Born和O′Brien法进行血小板聚集实验,可见马钱子碱(Brucine,B)和士的宁(Strychnine,S)各10^(-5)～10^(-3)mol/L明显抑制ADP、Epi和Thrombin诱导的人血小板聚集作用,呈剂量依赖性。②用Fura-2/AM检测B对Thrombin诱导人血小板内游离钙[Ca^(2+)]i的变化。结果:静息[Ca^(2+)]i值为111.14±11.71nmol/L(n=12)。Thrombin诱导的[Ca^(2+)]i峰值为1015±36.38nmol/L。B10^(-4)、10^(-3)mol/L分别使Thrombin诱导的[Ca^(2+)]i值下降至328.68±17.81和94.17±4.9nmol/L。在EGTA螯合血小板外钙[Ca^(2+)]o情况下,Thrombin诱导的[Ca^(2+)]i峰值为218.55±56.2nmol/L与静息[Ca^(2+)]i相比具有非常显著差异(P<0.01),提示Thrombin促血小板内钙释放;B10^(-4)、10^(-3)mol/L分别使其[Ca^(2+)]i值下降至89.93±6.31和75.49±4.37nmol/L,上述结果提示:B抑制Thrombin的促外钙内流及内钙释放,呈剂量依赖性。①The platelet aggregation was tested with the method of Born and O'Brien. It was shown that brucine(B) and strychnine(S) at the concetrations of 10^(-5)～10^(-3) mol/L could inhibit human platelet aggregation induced by ADP,Epi and thrombin which showed dose-dependence. ②The measurement of intracellular free Ca^(2+) Concentration in human platelet with Fure-2/AM. The data showed that the resting [Ca^(2+)]i was 111. 14± 11. 71 nmol/L(n=12). Thrombin could markedly enhance [Ca^(2+)]i to 1015. 62±36. 38nmol/L and this could be prevented or reversed by brucine 10^(-4) or 10^(-3)mol/L. After the extracellular Ca^(2+) in human platelet had been chelated by EGTA, thrombin 1U/ml induced [Ca^(2+)]i to 218. 55±56. 2nmol/L,the difference was significant as compared with the resting [Ca^(2+)]i (P<0. 01). This was related to intracellular Ca^(2+) mobilization and could be also reversed significantly by brucine 10^(-4) and 10^(-3) mol/L(P<0. 01). These results suggest that the inhibitory effect of brucine on extrcellular Ca^(2+) influx and intracellular Ca^(2+) mobilization induced by thrombin 1U/ml may show dose-dependence.

关 键 词：马钱子碱 士的宁 血小板聚集% 二磷酸腺苷 肾上腺素 凝血酶 钙螯合剂 FURA-2/AM 人血小板内游离钙浓度 

分 类 号：R[医药卫生]