中国胃癌高发县鱼露的致突/致癌性研究  

MUTAGENICITY AND CARCSNOGENICITY OF FISH SAUCE FROM A COUNTY WITH THE HIGH RISK FOR GASTRIC CANCER IN CHINA

作　　者：邓大君 张汝黻 陈跃 陈重升 金山 朱少侠 

机构地区：[1]北京市肿瘤防治研究所病因室北京

出　　处：《癌变．畸变．突变》1991年第1期13-17,60-61+64,共8页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：通过生物学短期试验和动物诱癌实验,我们研究了我国胃癌高发区福建长乐县(男性胃癌标化死亡率达113.20/10~5)鱼露的致突致癌作用。结果表明,鱼露经亚硝化后其乙酸乙酯萃取物具有明显的直接诱发S.typhimurum TA100回变、诱发V79细胞姐妹染色单体交换(SCE)和微核:诱发大肠杆菌PQ37SOS、使小牛胸腺DNA烷化的作用,并且能诱导人正常胃粘膜细胞DNA程序外合成(UDS),给新生Wistar大鼠灌胃后第四周和第十六周分别发现能诱发腺胃粘膜不典型增生(5/5)和腺胃癌(1/5);而未经亚硝化鱼露无此作用或作用很弱。进一步分析发现亚硝化鱼露中有N-亚硝酸胺(30,9-6.04μm),这类直接致灾致癌物的存在可能是亚硝化鱼露致突致癌作用的原因。长期食用鱼露可能与当地胃癌高发有关。The mutagenicity and carcinogenicity of fish sauce (FS) sample from Changle county, a high gastric cancer mortality ( 113.20/105 ) area, were investigated with the biologic short-term tests and laboratory animal experiment. The results showed that the extract of FS was marked direct mutagenic toward S. typhimurium TA100, induced high sister chromatid exchanges (SCE) and micronucleus (MN) in V79 cells after nitrosation with sodium nitrite. But the non-nitrosated FS did not. The nitrosated fish sauce (NFS) also induced SOS in E. coli PQ37 and alkylation of calf-thymus DNA. The potency of NFS to induce unscheduled DNA synthesis(UDS) in human normal gastric mucosal cells was increased about five fold compared with FS. When the NFS extract was given to newborn rats by gavage, dysplasia and adenocarcinoma were induced in the glandular stomach in the 4th and 16 th experimental week respectively. N-nitrosamides were also found in NFS, which may account for the mutagenicity and carcinogenicity of NFS. It is indicated that FS, a traditional daily eaten seasoning, may contribute to the causes of the high gastric cancer mortality for the local residents.

关 键 词：胃癌病因 亚硝酰胺 鱼露 致突变 致胃癌 人胃粘膜细胞 

分 类 号：R73[医药卫生—肿瘤]