THE EXPRESSION OF PROTOONCOGENES IN HUMAN GASTRIC CANCER CELL LINES AND PRIMARY GASTRIC CANCERS DEFINED BY IN SITU HYBRIDIZATION  

作　　者：陈季 王敦瑞 汪肖钢 于丽莉 陈诗书 

机构地区：[1]Shanghai Institute of Digestive Disease, SSMU [2]Laboratory of Molecular Biology, Department of Biochemistry, College of Basic Medical Sciences, SSMU, Shanghai

出　　处：《Medical Bulletin of Shanghai Jiaotong University》1990年第1期56-62,共7页上海交通大学学报（医学英文版）

摘　　要：The expression of several protooncogenes was detected in three different differentiated gastric cancer cell lines (MKN28, MKN45, SGC7901) and 5 specimens from 5 human primary gastric cancers at cellular level by in situ hybridization. The data indicated that there were high levels of c-Ha-ras expression in both cell lines and tumor tissues as compared with control samples. But the transcripts of c-myc and c-erbB were rather low. The results suggested that Ha-ras be involved in or associated with the gastric cancer. There were another two interesting findings: (1) The levels of c-Ha-ras was different among the individual cancer cells within a single gastric cnacer. (2) Some inflammatory cells in the tumor tissuse and normal tissues adjacent to the cancer displayed high level of c-Ha-ras expression. Those two phenomena are worthwhile for further study.The expression of several protooncogenes was detected in three different differentiated gastric cancer cell lines (MKN28, MKN45, SGC7901) and 5 specimens from 5 human primary gastric cancers at cellular level by in situ hybridization. The data indicated that there were high levels of c-Ha-ras expression in both cell lines and tumor tissues as compared with control samples. But the transcripts of c-myc and c-erbB were rather low. The results suggested that Ha-ras be involved in or associated with the gastric cancer. There were another two interesting findings: (1) The levels of c-Ha-ras was different among the individual cancer cells within a single gastric cnacer. (2) Some inflammatory cells in the tumor tissuse and normal tissues adjacent to the cancer displayed high level of c-Ha-ras expression. Those two phenomena are worthwhile for further study.

关 键 词：ONCOGENE GASTRIC cancer in SITU HYBRIDIZATION 

分 类 号：R[医药卫生]