应用DNA体外PCR扩增技术对地贫症进行产前基因诊断  

THE APPLICATION OF DNA AMPLIFICATION IN VITRO WITH POLYMERASE CHAIN REACTION TO THE PRENATAL GENE DIAGNOSIS OF THALASSEMIA

作　　者：张树球[1] 黄运忠[1] 钟鸣[1] 罗艳红[1] 卢彩珍[1] 李民[1] 陈惠勤[2] 韦红卫[2] 王利民[2] 秦明丽[2] 张慕洁 

机构地区：[1]右江民族医学院生化教研室 [2]右江民族医学院妇产科教研室 [3]右江民族医学院儿科教研室

出　　处：《右江民族医学院学报》1990年第3期41-44,共4页Journal of Youjiang Medical University for Nationalities

摘　　要：应用基因工程技术中最新的方法—DNA体外PCR扩增技术,对4例正常人和4例地贫患者的胎儿绒毛DNA中的α和β珠蛋白基因片段进行扩增成功,并对一例Hb Bart’s水肿胎儿进行了基因诊断。本法最大优点为迅速、灵敏、简便,且不需同位素标记探针,适用于边远地区进行地贫症产前基因诊断。We are successfully applied the recent method of gene enginering technique--DNA amplification in vitro with polymerase chain reaction to the gene piece of the α-and β-globin in fetal chorial villus DNA of 4 normal persons and 4 cases of thalassemia. The gene diagnosis for a hydropic fetus of Hb Bart 's was made. This method does not require isotope labelling probe. It is a rapid, highly sensitive and simple method which is provides for the prenatal gene diagnosis of the patient with thalassemia on the mountainous area.

关 键 词：基因 脱氧核糖核酸聚合酶类 地中海贫血 

分 类 号：R[医药卫生]