Genomic imbalances in esophageal carcinoma cell lines involve Wnt pathway genes  

作　　者：Jacqueline Brown Hannelie Bothma Robin Veale Pascale Willem 

机构地区：[1]National Health Laboratory Services and University of the Witwatersrand [2]School of Molecular and Cell Biology,University of the Witwatersrand

出　　处：《World Journal of Gastroenterology》2011年第24期2909-2923,共15页世界胃肠病学杂志（英文版）

摘　　要：AIM： To identify molecular markers shared across South African esophageal squamous cell carcinoma （ESCC） cell lines using o/togenetics, fluorescence in situ hybridization （FISH） and single nucleotide polymorphism （SNP） array copy number analysis. METHODS： We used conventional cytogenetics, FISH, and multicolor FISH to characterize the chromosomal rearrangements of five ESCC cell lines established in South Africa. The whole genome copy number profile was established from 250K SNP arrays, and data was analyzed with the CNAT 4.0 and GISTIC software. tions involved the following chromosomal regions and genes： 11q13.3 （CCND1, FGF3, FGF4, FGF19, MYEOV）, 8q24.21（C-MYC, FAM84B）, 11q22.1-q22.3 （B[RC2, BIRC3）, 5p15.2 （CTNND2）, 3qll.2-q12.2 （MINA） and 18p11.32 （TYMS, YES1）. The significant deletions included 1p31.2-p31.1 （CTH, GADD45a, DIRAS3）, 2q22.1 （LRPIB）, 3p12.1-p14.2 （FHIT）, 4q22.1-q32.1 （CASP6, SMAD1）, 8p23.2-q11.1 （BNIP3L） and 18q21.1-q21.2 （SMAD4, DCC）. The 3p11.2 translocation breakpoint was shared across four cell lines, supporting a role for genes involved at this site, in particular, the EPHA3 gene which has previously been reported to be deleted in ESCC.CONCLUSION： The finding that a significant number of genes that were amplified （FGF3, FGF4, FGF19, CCND1 and C-MYC） or deleted （SFRP2 gene） are involved in the Wnt and fibroblast growth factor signaling pathways, suggests that these pathways may be activated in these cell lines.AIM:To identify molecular markers shared across South African esophageal squamous cell carcinoma(ESCC) cell lines using cytogenetics,fluorescence in situ hybridization(FISH) and single nucleotide polymorphism(SNP) array copy number analysis.METHODS:We used conventional cytogenetics,FISH,and multicolor FISH to characterize the chromosomal rearrangements of five ESCC cell lines established in South Africa.The whole genome copy number profile was established from 250K SNP arrays,and data was analyzed with the CNAT 4.0 and GISTIC software.RESULTS:We detected common translocation breakpoints involving chromosomes 1p11-12 and 3p11.2,the latter correlated with the deletion,or interruption of the EPHA3 gene.The most significant amplifica-tions involved the following chromosomal regions and genes:11q13.3(CCND1,FGF3,FGF4,FGF19,MYEOV),8q24.21(C-MYC,FAM84B),11q22.1-q22.3(BIRC2,BIRC3),5p15.2(CTNND2),3q11.2-q12.2(MINA) and 18p11.32(TYMS,YES1).The significant deletions included 1p31.2-p31.1(CTH,GADD45α,DIRAS3),2q22.1(LRP1B),3p12.1-p14.2(FHIT),4q22.1-q32.1(CASP6,SMAD1),8p23.2-q11.1(BNIP3L) and 18q21.1-q21.2(SMAD4,DCC).The 3p11.2 translocation breakpoint was shared across four cell lines,supporting a role for genes involved at this site,in particular,the EPHA3 gene which has previously been reported to be deleted in ESCC.CONCLUSION:The finding that a significant number of genes that were amplified(FGF3,FGF4,FGF19,CCND1 and C-MYC) or deleted(SFRP2 gene) are involved in the Wnt and fibroblast growth factor signaling pathways,suggests that these pathways may be activated in these cell lines.

关 键 词：ESOPHAGUS CANCER Single nucleotide polymorphism arrays Fluorescent in situ hybridization 

分 类 号：R735.1[医药卫生—肿瘤]