普洱茶水提物对巨噬细胞分泌肿瘤坏死因子-α的影响  

Effect of Pu-erh Tea Extract on Secretion of Tumor Necrosis Factor-α by Macrophages

作　　者：李春磊[1,2] 王宣军 宋爽[2,3] 李冬青[1,2] 师思[2,4] 郝淑美[3] 盛军 

机构地区：[1]长春理工大学,长春130022 [2]云南省茶深加工工程技术研究中心,昆明650201 [3]吉林大学生命科学学院,长春130012 [4]云南大学,昆明650091

出　　处：《中国生物制品学杂志》2011年第11期1293-1295,1302,共4页Chinese Journal of Biologicals

摘　　要：目的探讨普洱茶水提物对巨噬细胞分泌肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)的影响。方法以佛波酯处理THP-1细胞48 h,分化得到巨噬细胞,以其为炎症细胞模型,用不同浓度的普洱茶水提物(125、250μg/ml)与终浓度为100 EU/ml的脂多糖的混合物作用于巨噬细胞,ELISA法检测细胞培养液中TNF-α的含量。结果普洱茶水提物能有效抑制巨噬细胞炎症因子TNF-α的分泌,且浓度为250μg/ml的普洱茶水提物对TNF-α分泌的抑制作用强于浓度为125μg/ml的普洱茶水提物,呈剂量依赖性。结论普洱茶水提物对巨噬细胞炎症因子TNF-α的分泌具有抑制作用。这种效果与已知的绿茶抗炎的主要成分EGCG无关。Objective To investigate the effect of Pu-erh tea extract on secretion of tumor necrosis factor-or （TNF-α） by maerophages. Methods Macrophages were differentiated from THP-1 cells after treatment with phorbol ester and used as the model of inflammatory ceils, which were treated by the mixture of lipopolysaccharide （LPS） at a final concentration of 100 EU/ml with Pu- erh tea extract at various concentrations （125 and 250 μg/ml） and determined for TNF-α content in cell culture by ELISA. Restilts Pu-erh tea extract effectively inhibited the secretion of TNF-α by macrophages. However, the inhibitory effect of 250 μg / ml Pu-erh tea extract was stronger than that of 125 Ixg/ml extract. Conclusion Pu-erh tea extract showed dose-dependent inhibitory ef- fect on secretion of TNF-α by maerophages, and the effect was unassociated with EGCG, one of the major anti-inflammatory components of green tea.

关 键 词：普洱茶 炎症 巨噬细胞 肿瘤坏死因子-αTHP-1细胞 

分 类 号：S571.1[农业科学—茶叶生产加工] Q51[农业科学—作物学]