雷帕霉素对胃癌细胞增殖与凋亡的影响及机制  

Effects of rapamycin on proliferation and apoptosis of gastric cancer cells and its mechanism

作　　者：姚春[1] 邵力伟[1] 刘锦修[1] 伍龙[2] 

机构地区：[1]武汉大学广慈医院（武汉市第五医院）武汉市血液病研究所,430050 [2]武汉大学人民医院肿瘤科

出　　处：《中华实验外科杂志》2011年第12期2152-2154,共3页Chinese Journal of Experimental Surgery

基　　金：基金项目：湖北省卫生厅科研基金资助项目（JX5823）;武汉市卫生局资助项目（WX-08C17）

摘　　要：目的观察雷帕霉素对人胃癌细胞株SGC-901和MKN45的增殖、凋亡以及侵袭能力的影响并探讨其机制。方法采用不同浓度的霄帕霉素（5、10、20、40nmol／L），噻唑蓝（MTT）和流式细胞术分别检测SGC-7901和MKN45细胞的增殖和凋亡、Transwell检测胃癌细胞的侵袭能力、逆转录．聚合酶链反应（RT-PCR）和Westernblot检测Survivin和roTOR的mRNA和蛋白的表达。结果不同浓度的雷帕霉素可以抑制胃癌细胞株SGC-7901和MKN45的增殖并诱导其发生凋亡，同时可降低其体外侵袭能力，其作用具有剂量依赖性。RT—PCR和Westernblot检测显示胃癌细胞中rnTOR和Survivin的mRNA和蛋白表达存雷帕霉素作用后受到抑制。结论雷帕霉素可以体外抑制胃癌细胞的增殖和侵袭并且诱导其发生凋亡，其可能是通过抑制roTOR和Survivin的表达来发挥作用的。Objective To observe the effects of rapamycin on proliferation, apoptosis and invasion of human gastric cancer cell lines, SGC-7901 and MKN 45, and its mechanisms. Methods Different concentrations of rapamycin （5, 10, 20, 40 nmol/L） were used. Methyl thiazol tetrazolium （MTF） and flow cytometry assays were used to detect the proliferation and apoptosis of SGC-7901 and MKN 45 ceils. Transwell assay was used to observe the invasive ability of gastric cancer cells. By using reverse transcription- polymerase chain reaction （RT-PCR） and Western blotting, the mRNA and protein expression of mTOR and Survivin was examined. Results Our data showed that rapamycin could inhibit proliferation and invasive ability of SGC-7901 and MKN 45 cells, and induce apoptosis of these two gastric cancer cell lines in a dose-dependent manner. The mRNA and protein expression of roTOR and Survivin was suppressed by rapamycin. Conclusion Rapamycin could inhibit proliferation, invasive ability and induce apoptosis of human gastric cancer cells in vitroprobably by inhibiting the expression of roTOR and Survivin.

关 键 词：雷帕霉素 MTOR 胃癌 SURVIVIN 

分 类 号：R735.2[医药卫生—肿瘤]