二烯丙基二硫对人胃癌SGC7901细胞Au-rora-A、Aurora-B表达的影响  

Effects of Diallyl Disulfide on Expressions of Aurora-A and Aurora-B in Human Gastric Cancer Cell Line SGC7901

作　　者：李振丰[1] 凌晖[1] 陈真伟[1] 段海瑞[1] 文玲[1] 胡程[1] 

机构地区：[1]南华大学肿瘤研究所

出　　处：《肿瘤防治研究》2011年第11期1241-1244,共4页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目(81071966);南华大学博士科研启动基金资助项目(2007XQD26)

摘　　要：目的探讨二烯丙基二硫(DADS)对人胃癌SGC7901细胞中Aurora-A、Aurora-B表达的影响。方法体外培养SGC7901细胞,采用RT-PCR、Western blot和免疫细胞化学分析DADS对SGC7901细胞中Aurora-A、Aurora-B表达的影响。结果 RT-PCR显示用15 mg/L DADS处理SGC7901细胞24、48 h后,Aurora-A、Aurora-B mRNA表达明显下调(P<0.05);Western blot显示用15 mg/L DADS处理SGC7901细胞24、48 h后,Aurora-A、Aurora-B蛋白表达水平呈时间依赖性下降(P<0.05);免疫细胞化学显示用15 mg/L DADS处理SGC7901细胞24、48 h后,Aurora-A和Aurora-B蛋白表达明显降低。结论 DADS可从基因和蛋白水平抑制SGC7901细胞Aurora-A、Aurora-B的表达。Objective To investigate the effects of diallyl disulfide（DADS） on the expressions of Aurora-A and Aurora-B of human gastric cancer cell line SGC7901.Methods SGC7901 cells were cultured in vitro,and the expressions of Aurora-A and Aurora-B of human gastric cancer cell line SGC7901 were measured by RT-PCR,Western blot and immunocytochemistry.Results Treated with 15 mg/L DADS for 24 h and 48 h,the expressions and proteins of Aurora-A and Aurora-B mRNA in SGC7901 cells reduced（P〈0.05） in a time-dependent manner.Conclusion DADS down-regulates mRNA and protein expressions of Aurora-A and Aurora-B in gastric cancer cells.

关 键 词：胃癌 二烯丙基二硫 AURORA-A AURORA-B 

分 类 号：R364.1[医药卫生—病理学] R735.2[医药卫生—基础医学]