重组人γ-干扰素基因工程菌发酵工艺优化  

Optimization of Fermentation Technology of Recombinant Human Interferon-γ

作　　者：许崇利[1,2] 欧阳红生[2] 许崇波[3] 张艳平[3] 

机构地区：[1]吉林化工学院环境与生物工程学院,吉林吉林132022 [2]吉林大学畜牧兽医学院,长春130062 [3]大连大学医学院,辽宁大连116622

出　　处：《中国兽药杂志》2011年第11期26-30,共5页Chinese Journal of Veterinary Drug

基　　金：国家自然科学基金项目(30972188)

摘　　要：研究不同类型培养基、诱导时间、菌体密度和不同补料对rhIFN-γ表达的影响,并运用高密度发酵工艺对rhIFN-γ的产量进行优化,目的蛋白表达量从4 000 mg/L提高到20 000 mg/L以上,发酵产量提高了5倍,大大提高了效率。同时为验证工艺的稳定性,将优化的发酵条件进行连续三批重复发酵实验。结果表明该优化条件具有重复性,表达稳定,维持在40%左右,从而为rhIFN-γ大规模生产奠定了良好的基础。The expression level of rhIFN-γ was influenced by the different medium,induction time,cell density and feeding batch.The production of rhIFN-γ was optimized by high density fermentation.The expression level of rhIFN-γ was increased from 4 000 mg/L to over 20 000 mg/L,the fermentation yield was increased by 5 times.In order to confirm the technical stability,a three-time repeated fermentation was performed.The result indicated that the optimal conditions had the repeatability and the expression level remained 40% or so.This fermentation technology established a good foundation for the rhIFN-γ mass production.

关 键 词：重组人Γ-干扰素 发酵 优化 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]