锁阳中黄酮的抗氧化功能研究  

Antioxidant Function of Flavonoids from Cynomorium songaricum Rupr.

作　　者：郭豫[1] 赵江燕[1] 栾娜[1] 张静[1] 李迪[1] 

机构地区：[1]北京联合大学应用文理学院,北京100191

出　　处：《安徽农业科学》2011年第32期19763-19764,19780,共3页Journal of Anhui Agricultural Sciences

基　　金：北京市教委科技计划项目(KM200811417002)

摘　　要：[目的]探索锁阳黄酮对衰老小鼠机体抗氧化功能的影响。[方法]采用9月龄雌性昆明小鼠,按体重随机分为4组。空白对照组每日灌胃无菌水;将制备的锁阳提取物干粉以无菌水制成溶液,低、中、高剂量组,按总黄酮计,分别按(BW)0.08、0.17、0.50 g/kg的量灌胃,连续灌胃54 d,按南京建成试剂盒说明分别测定超氧化物歧化酶(SOD)、丙二醛(MDA)、心肌脂褐质水平变化。[结果]与空白对照组比较,各剂量组SOD活性均有明显的提高,低、高剂量组分别提高了18.6%和19.5%(P<0.05),中剂量组提高了22.7%,差异显著(P<0.01);各剂量组MDA含量均减少,中剂量组减少了15.0%,高剂量组减少了26.0%,差异显著(P<0.01);各剂量组心肌脂褐质含量均减少,中、高剂量组分别减少了11.2%和9.7%,差异显著(P<0.05)。[结论]超声提取的锁阳中的黄酮具有抗氧化功能。[Objective] The aim was to explore the effects of flavonoids extracted from Cynomorium Songaricum Rupr.on the antioxidant function of aging mice.[Method]9-month-old female Kunming mice were divided randomly into four groups.In control group,mice were given sterile water by gavage;in the low,medium and high dose groups,mice were given solutions of Cynomorium Songaricum Rupr.extract powder and sterile water by gavage for 54 d,with the flavonoids doses of BW 0.08,0.17,and 0.50 g/kg,respectively.Changes of SOD,MDA,and myocardial lipofuscin were determined according to the instructions of kits.[Result] Compared with the control group,the activity of SOD increased significantly by 18.6%（P〈0.05）,19.5%（P〈0.05） and 22.7%（P〈0.01） in low,high and medium dose groups,respectively.MDA content reduced in three dose groups,which were 15.0%（P〈0.05）in medium dose group and 26.0%（P〈0.01）in high dose group.Myocardial lipofuscin content also decreased,in three dose groups,which were 11.2% and 9.7% in medium dose group and high dose group,respectively.[Conclusion] Flavonoids extracted from Cynomorium Songaricum Rupr.by ultrasound had certain antioxidation activity.

关 键 词：锁阳(HERBA CYNOMORII) 黄酮 抗氧化 

分 类 号：Q946.8[生物学—植物学]