高效液相色谱分析乳清蛋白方法研究  

Study on Analysis of Whey Protein by HPLC

作　　者：赵大伟 刘玲[2] 肖诗英[2] 

机构地区：[1]深圳九恒印刷设备器材有限公司,北京100037 [2]湖北工业大学化学与环境工程学院,湖北武汉430068

出　　处：《食品研究与开发》2011年第11期93-95,共3页Food Research and Development

摘　　要：建立测定乳清蛋白中α-乳白蛋白和β-乳球蛋白含量的高效液相色谱分析方法,采用Agilent的ZORBOX Eclipse XDB-C8色谱柱(150 mm×4.6 mm),流动相A为10%乙腈中含0.1%三氟乙酸,流动相B为90%乙腈中含0.1%三氟乙酸。采用梯度洗脱,流速为0.25 mL/min,二极管阵列检测器,检测波长214 nm,柱温40℃。外标法定量,α-La和β-Lg两种组分线性关系良好,相关系数分别为0.993 1和0.990 9,检测限为3μg/mL、8μg/mL。A method was established for the determination of α-lactalbumin and β-lactoglobulin by reverse-phase HPLC.Separation and quantification was achieved by using a Agilent ZORBOX Eclipse XDB-C8 column（150 mm×4.6 mm）,mobile phase of A 0.1 % TFA in 10 % acetonitrile and B 0.1 % TFA in 90 % acetonitrile,with gradient eluention.Mobilephase at a flow-rate of 0.25 mL/min,temperature 40 ℃ and monitored with photodiode array detector at 214 nm.The quantified analysis of external standard calibrationcurves was used.There were good linear correlations about the two components.The correlation coefficients were 0.993 1 and 0.990 9.The detection limits were 3 μg/mL and 8 μg/mL.

关 键 词：乳清蛋白 Α-乳白蛋白 Β-乳球蛋白 高效液相色谱 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]