南极产低温脂肪酶菌株Psychrobacter sp. G发酵条件优化研究  

Study on Optimization Fermentation Conditions for Producing Low-temperature Lipase by Psychrobacter sp. G

作　　者：衣丹[1] 林学政[1] 沈继红[1] 徐国英[1] 王能飞[1] 王帅[1] 

机构地区：[1]国家海洋局第一海洋研究所,山东青岛266061

出　　处：《海洋科学进展》2011年第4期506-511,共6页Advances in Marine Science

基　　金：国家高技术研究发展计划项目-极地特殊海洋微生物资源利用的关键技术研究资助课题(2007AA091905)

摘　　要：从南极第24次科学考察采集的样品中分离出产低温脂肪酶菌株Psychrobacter sp.G,通过Plackett-Burman法及响应面法对其产脂肪酶条件进行优化,得出最适条件为:蛋白胨0.75%、酵母粉0.2%、NaCl 2%、Mg-SO40.1%、(NH4)2SO40.1%、K2HPO40.2%、Tween-80 1.5%、培养温度15℃、培养时间72h、装液量150mL/500mL、振荡转速150r/min。在此条件下发酵的脂肪酶活力为16.5U/mL。Psychrobacter sp. G is isolated from the samples collected during the 24th Antarctic Scientific Expedition of China. The Plackett-Burman design and response surface method are used to rapidly optimize the lipase production conditions. The optimal conditions for lipase production are as the followings： tryptone 0.75 %, yeast powder 0.2%, NaCl 2%, MgSO4 0.1%, （NH4）2SO4 0.1%, K2HPO4 0.2%, Tween- 80 1.5 %, the culture temperature 15 ℃, the culture period 72 h, 150 mL fermenting liquor in 500 mL flask, and the shaking frequence 150 r/min. The highest production of low-temperature lipase is 16.5 U/ mL.

关 键 词：低温脂肪酶 Plackett--Burman法 响应面分析 

分 类 号：Q933[生物学—微生物学]