Plumbagin对人类大细胞肺癌NL9980细胞系抑癌作用的研究  

Research on the anti-tumor effect of Plumbagin against human large cell lung cancer cell line NL9980

作　　者：于涛[1] 马力[1] 朱彧[1] 祖玲玲[1] 王竞[1] 

机构地区：[1]天津医科大学总医院肿瘤内科天津市肺癌转移与肿瘤微环境重点实验室天津市肺癌研究所,300052

出　　处：《肿瘤研究与临床》2011年第11期725-728,732,共5页Cancer Research and Clinic

摘　　要：目的研究Plumbagin对人类大细胞肺癌NL9980细胞系的抗肿瘤作用并探索其机制。方法不同浓度Plumbagin处理NL9980细胞系，采用MTT法观察其对肿瘤细胞增殖的影响，确定药物IC50，采用流式细胞术检测对细胞系诱导凋亡的作用，采用Boyden小室侵袭实验检测对体外侵袭力的抑制作用；以IC50 Plumbagin处理NL9980细胞系，于处理后6、24及48h收获细胞，以实时定量PCR方法检测bcl-2、bax、VEGF和CYCD1等基因的mRNA表达变化。结果MTT法显示Plumbagin明显抑制NL9980细胞系的细胞增殖，IC50为7．5μmol／L；Plumbagin对NL9980细胞系具有体外诱导凋亡作用，且明显抑制其体外侵袭能力，Bodyen小室侵袭实验检测对照组和Plumbagin组平均穿膜细胞数分别为161．59±47．32和26．58±9．07。Plumbagin处理NL9980细胞系后不同时间点检测结果显示，bcl-2、VEGF和CYCD1基因表达均逐渐减低，bax基因表达逐渐增高，组间差异有统计学意义（均P〈0．05）。结论Plumbagin对大细胞肺癌NL9980细胞系具有明确的抗肿瘤作用。Plumbagin可能通过多种作用机制发挥其抑癌作用，显示了其成为抗大细胞肺癌药物的前景。Objective To investigate the anti-tumor effect and mechanism of Plumbagin against human large cell lung cancer cell line NL9980. Methods Plumbagin in different concentrations were set up to administrate NL9980 cell line. MTr test was performed to detect the inhibiting effects against tumor proliferation and IC50 concentration was identified. The influence of tumor apoptosis was observed by flow cytometry and the inhibiting effect against invasive ability was detected by Boyden chamber test. IC50 group and control group were set up and tumor cells were harvested 6 h, 24 h and 48 h after administration. Realtime PCR was used to detect mRNA expression changes of bcl-2, bax, VEGF and CYCD1. Results MTr test showed obvious inhibiting effects of proliferation in NL9980 cell line and IC50 was 7.5 μmol/L. Plumbagin induced the apoptosis of tumor cells and showed a significant anti-invasive effect. Mean membrane-spanning cells were 161.59±47.32 and 26.58±9.07 in control group and IC50 group correspondingly. Down-regulations of bel-2, VEGF and CYCD1 were detected and expression of bax gene increased. The effect of Plumbagin showed obvious time dependance （P 〈0.05）. Conclusion Plumbagin has displayed the powerful antitumor effects against NL9980 cell line and it might work via multiple pathways, Plumbangin exhibits the prospect of being an effective drug against large cell lung cancer.

关 键 词：肺肿瘤 癌 大细胞 PLUMBAGIN 增殖 侵袭 凋亡 

分 类 号：R734.2[医药卫生—肿瘤]