转CpTI基因大米在五指山小型猪体内消化稳定性的初步研究  

In vivo digestibility of rice genetically modified with CpTI in WZS mini-pig

作　　者：黄琼[1] 刘海波[1] 支媛[1] 高芃[1] 于州[1] 刘珊[1] 徐海滨[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100021

出　　处：《卫生研究》2011年第6期680-683,共4页Journal of Hygiene Research

基　　金：国家重点基础研究发展(973)计划(No.2007CB109207);转基因生物新品种培育科技重大专项(No.2008ZX08011-005)

摘　　要：目的建立五指山小型猪在体消化稳定性模型,评价转CpTI大米的胃肠消化稳定性。方法在五指山成年猪体内同时安置胃、肠瘘管,通过经口喂饲途径,以大豆作为阳性对照,测定不同时间点消化液的pH,采用蛋白凝胶电泳法分析测定不同时间点消化产物中的蛋白片段,比较转CpTI大米和亲本大米消化产物的区别。结果给样后胃液pH迅速中和至6.0左右,以后逐步降低,在4～6h后又开始回升,大豆引起胃液pH下降和回升时间均较米粉晚;13×103、17×103、34×103和50×103是大豆中几种相对消化稳定的蛋白片段,在5～6h的胃肠液中仍然能检测出高表达,其中50×103、34×103、17×103三个蛋白片段呈现胃液、肠液消化稳定性,而13kD呈现胃液消化稳定性;米粉给样0.25h后胃液中未检出任何耐受蛋白酶消化的片段或亚基,而亲本明恢86米粉和转CpTI基因米粉比较,无论是胃液或肠液中蛋白消化情况均无明显区别。结论利用五指山小型猪建立的在体消化稳定性模型是可行的,转CpTI基因米粉与亲本米粉在五指山小型猪胃液、肠液中的消化稳定性具有等同性。Objective To establish a stable in vivo gastrointestinal model of WZS mini-pig to evaluate the digestibility of rice genetically modified with CpTI（Cowpea Trypsin Inhibitor）.Methods Three WZS mini-pigs were surgically fitted with O-stomach cannula and T-ileum cannula,and fed with soybean（positive control）,CpTI rice and its parental rice meals.The pH value of gastric and intestinal fluid was monitored at different time points.The digested protein products were measured with protein gel electrophoresis at different time points.Results The pH value of gastric contents was rapidly neutralized by the meal to approximately 6.0,then the pH was reduced by HCl secretion,and it subsequently was increased after 4-6 hours.Compared with rice,the increase or decrease of pH after soybean being fed was later.Soybean protein segments 13kD,17kD,34kD and 50kD could be highly detected in gastric and intestinal fluid at 5-6h after soybean being introduced.The segment 13kD was digested in intestine.However,no any protein segment was found in the gastric fluid 0.25h after rice being fed.There was no any difference in digestibility between the rice genetically modified with CpTI and its parental rice.Conclusion It is practicable to establish a in vivo model of WZS mini-pig for digestibility.The digestibility of CpTI rice and its parental rice in gastric and intestinal tract in vivo is equivalent.

关 键 词：转CpTI基因大米 消化稳定性 五指山小型猪 食物过敏 实质等同性 

分 类 号：TS201.6[轻工技术与工程—食品科学] R155.52[轻工技术与工程—食品科学与工程]