超高效液相色谱-质谱法测定动物性膳食中全氟辛烷磺酸和全氟辛酸  

Quantitative determination of perfluorooctanesulfonate and perfluorooctanoic acid in animal food by UPLC-MS/MS

作　　者：刘嘉颖[1,2] 王雨昕[1] 李敬光[1] 赵云峰[1] 吴永宁[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100050 [2]北京市朝阳区疾病预防控制中心,北京100021

出　　处：《中国食品卫生杂志》2011年第6期539-543,共5页Chinese Journal of Food Hygiene

基　　金：国家自然科学基金(20607021)

摘　　要：目的建立三类动物性膳食中典型全氟有机化合物的超高效液相色谱-串联质谱(UPLC-MS/MS)测定方法。方法实验优化了样品提取方法和液相色谱质谱条件。样品经冷冻干燥后用甲醇提取,弱阴离子交换固相萃取柱净化;使用C18色谱柱,甲醇和2 mmol/L醋酸铵水溶液为流动相进行分离;采用ESI负离子源并在多反应监测模式(MRM)下进行测定。结果目标化合物的回收率为82%～115%,检测限分别为76～129 pg/g干重和88～319 pg/g干重。运用该方法分析了9份膳食样品,三类样品中均检出目标化合物。结论本方法简便快速、准确可靠,灵敏度足以满足动物性膳食样品的检测。Objective To establish a method for detecting perfluorooctanesulfonate（PFOS） and perfluorooctanoic acid（PFOA） in three kinds of animal food.Methods Samples were extracted by methanol and cleaned-up by SPE in a WAX column.The analytes were separated and detected on ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS） by C18 column with mobile phase of methanol and 2 mmol/L ammonium acetate aqueous solution,and performed with negative EST at multiple reaction monitoring（MRM） mode.Results The average recoveries were 82%~115%.The detection limits of PFOS were 76~129 pg/g dry weight and that of PFOA were 88~319 pg/g dry weight.Target compounds were confirmed successfully in 3 of 9 animal food samples.Conclusion The method is easy,robust and sensitive for detecting PFOS and PFOA in animal food.

关 键 词：全氟有机污染物 全氟辛烷磺酸 全氟辛酸 UPLC-MS/MS 食品污染物 食品安全 

分 类 号：TS207.3[轻工技术与工程—食品科学]