荧光衍生化法测定不同季节桑叶中1-脱氧野尻霉素的含量  

Determination of 1-deoxynojirimycin in Morus alba L.leaves of different seasons by fluorometric derivatization

作　　者：李继文[1,2] 郭晓燕[2] 王腾[2] 李翠清[2] 

机构地区：[1]北京化工大学化学工程学院,北京100029 [2]北京石油化工学院化学工程学院,北京102617

出　　处：《食品工业科技》2011年第12期462-464,467,共4页Science and Technology of Food Industry

基　　金：教育部科学技术研究重点项目(211003);北京市教育委员会科技发展计划面上项目(KM201110017002)

摘　　要：目的:建立测定桑叶中1-脱氧野尻霉素(1-DNJ)含量的方法,并比较不同季节桑叶中1-DNJ的含量。方法:采用高效液相色谱法测定,以芴甲氧羰酰氯(FMOC-Cl)为柱前衍生化试剂,对衍生化条件进行了优化;色谱柱为Kromasil C18柱(4.6mm×250mm,5μm),流动相为乙腈-0.1%醋酸(50∶50),流速1.0mL/min,柱温30℃,荧光检测器激发波长254nm,发射波长322nm。结果:1-DNJ在10～35mg/L范围内线性良好(r=0.99959),检测限为0.80mg/L(S/N=3),平均回收率为95.38%,相对标准偏差2.28%(n=5)。桑叶中1-DNJ的含量在不同生长季节间存在显著差异。结论:方法简便、快速、准确,适用于桑叶中1-DNJ含量的定量分析。Objective：To develop a high performance liquid chromatographic method for the determination of 1-deoxynojirimycin（DNJ）in Morus alba L. leaves and contrast the content of 1-DNJ in different seasons. Methods：The separation was performed with a Kromasil C18 column（4. 6mm×250mm,5μm）at 30℃ and the mobile phase consisted of acetonitrile-0. 1%aqueous acetic acid（50∶ 50）,at the rate of 1. 0mL/min. The 1-DNJ marked with 9-fluorenylmethyl chloroformate（FMOC-Cl）was detected at excitation wavelength of 254nm and emission wavelength of 322nm. Results：The calibration curve was linear over the concentration range from 10mg/L to 35mg/L（r=0. 99959）,the limit of detection was 0. 80mg/L（S/N=3）and the average recovery was 95. 38%（RSD=2. 28%）. The results showed that the content of 1-DNJ was significantly different in different seasons. Conclusion：This method was simple,rapid,and accurate and was suitable for the determination of 1-DNJ in Morus alba L. leaves.

关 键 词：桑叶 1-脱氧野尻霉素 高效液相色谱法 荧光衍生化 

分 类 号：TS207.3[轻工技术与工程—食品科学]