基于抗体微阵列的食源性病原体综合检测平台  

Comprehensive platform for detecting foodborne diseases pathogens based on antibody microarrays

作　　者：林荣生[1] 

机构地区：[1]厦门华厦职业学院食品药品安全检测中心,福建厦门361024

出　　处：《食品工业科技》2011年第12期489-493,共5页Science and Technology of Food Industry

摘　　要：针对超低浓度食源性病原体样本检测的需要,将表面沉积铁氰酸镍薄膜的微叉指电极与生物活化的微珠相结合,设计并实现了通过测定电化学阻抗变化的抗体微阵列食源性病原体综合检测平台样件。提出了电化学阻抗等效电路,并通过电路模型分析表明,生物活化的微珠、微叉指电极之间的电容、溶液的电阻均对电化学阻抗传感单元的输出特性有显著影响。以不同浓度的病原体为样本,完成了Escherichia coli O157∶H7为目标病原体的实验研究。实验结果表明,该微抗体阵列的检出极限低至1.0pg/mL,可满足超低浓度食源性病原体的检测需要。With the aim to solve the difficulty in detection of ultra-low level foodborne disease pathogens on the spot,a comprehensive antibody microarray platform for rapid detection of multiple foodborne disease pathogens had been designed and realized. By combination of interdigitated microelectrodes（IDMs）deposited with thin nickel hexacyanoferrate film and bioactivated microbeads,the electrochemical impedance（ECI）cell had been rendered with a good sensitivity. An equivalent circuit was proposed to analysis the effects from the parameters of the ECI cell. It was found the existence of the microbeads,capacitance between the IDMs,and resistance in the solution could influence of the impedance of the ECI cell. Witht Escherichia coli O157∶ H7 as the target pathogens,experiments were carried out with different concentration of target. The experimental results showed a good detection limitation at 1. 0pg/mL. It indicated that the antibody microarray platform was suitable for ultra-low level detection of foodborne disease pathogens.

关 键 词：食源性病原体 抗体微阵列 超低浓度 电化学阻抗 生物活化微珠 

分 类 号：TS207.3[轻工技术与工程—食品科学]