新疆卡拉库尔羊TNF-α基因的克隆及核苷酸序列分析  

Cloning and Nucleotide Sequence Analysis of TNF-α Gene of Karakul Sheep of Xinjiang

作　　者：贺艳艳[1] 潘辉[1] 刘书东[1] 左文斌[1] 李莲瑞[2] 

机构地区：[1]塔里木大学生命科学学院,新疆阿拉尔843300 [2]新疆生产建设兵团塔里木畜牧科技重点实验室/塔里木大学动物科学学院,新疆阿拉尔843300

出　　处：《塔里木大学学报》2011年第3期54-58,共5页Journal of Tarim University

摘　　要：根据普通绵羊的碱基序列和相关文献设计引物,用RT-PCR扩增并测定了卡拉库尔羊肿瘤坏死因子-α基因的cDNA序列。结果表明,TNF-αcDNA序列包含了一个705 bp的开放阅读框,通过GenBank中的Blastn序列比较分析,卡拉库尔羊的TNF-α基因与普通绵羊的同源性高达99.86%。这为将来制备卡拉库尔羊TNF-α基因探针及卡拉库尔羊分子免疫学的发展提供了理论依据。The prime was designed according to the DNA sequence of the ovis and relevant literature, the sequence of the kalakul＇s TNF - α gene was amplified by RT - PCR and sequenced. The result showed that the cDNA sequence of TNF - α contains a 705 bp of ORF. The comparison analysis of the fragment sequence with ovis through hlastn of GenBank, the homologies of the nucleotide sequence with ovis were 99.86 %. This research provide the scientific theoretical foundation on producing method of probe of kalakulg TNF - αand development of Molecular Immunology.

关 键 词：卡拉库尔羊 肿瘤坏死因子-Α 核苷酸序列分析 

分 类 号：S826[农业科学—畜牧学]