乙醇脱氢酶2基因的Arg47His变异与慢性乙型肝炎的关系  

Association between Arg47His mutation in the ADH2 gene and chronic hepatitis B

作　　者：范桂玲[1] 姚勇 姚丽[3] 贾崇奇[4] 

机构地区：[1]山东大学附属济南市传染病医院肝病科,济南250021 [2]山东单县人民医院,山东单县274300 [3]山东中医药大学第二附属医院,济南250012 [4]山东大学公共卫生学院,济南250012

出　　处：《山东大学学报（医学版）》2011年第11期101-104,共4页Journal of Shandong University:Health Sciences

摘　　要：目的探讨乙醇脱氢酶2(ADH2)基因的Arg47His变异与慢性乙型肝炎(慢乙肝)之间的关系。方法采用住院病例对照研究设计。以252例中国汉族慢乙肝患者为慢乙肝组,以223名中国汉族正常人为对照组。PCR-RFLP方法检测ADH2基因的Arg47His变异。运用F检验比较各组之间的均数差异;运用χ2分析各组之间的频数分布差异;运用单因素及多因素Logistic回归分析ADH2基因Arg47His变异与慢性肝炎之间的关系。结果 ADH2基因Arg47His变异Arg与His等位基因频率在慢乙肝组(37.3%、62.7%)与对照组(28.5%、71.5%)比较,差异有统计学意义(P=0.005);Arg/Arg、Arg/His、His/His基因型频率在慢乙肝组(13.1%、48.4%、38.5%)与对照组(7.2%、42.6%、50.2%)比较,差异有统计学意义(P=0.014)。以His/His基因型为参照组,Arg/His与Arg/Arg基因型单因素Logistic回归分析OR分别为1.48(95%CI:1.01～2.17)与2.38(95%CI:1.24～4.59)。调整性别、年龄、吸烟、饮酒、肝炎家族史后,其OR分别为1.46(95%CI:0.97～2.19)与2.51(95%CI:1.25～5.02)。结论 ADH2基因Arg47His变异野生型基因类型可能是中国汉族人群慢性乙型肝炎发病的重要危险因素之一。Objective To study the relationship between the ethanol dehydrogenase 2（ADH2） gene′s Arg47His mutation and chronic hepatitis B.Method The ADH2 gene Arg47His mutation was analyzed with PCR-RFLPin in 252 Chinese Han nationality chronic hepatitis B patients（CHB Group） and 223 healthy individuals（Control group）.Logistic regression analysis was performed to explore the relationship between the ADH2 gene Arg47His mutation and chronic hepatitis B.Result The distributions of the ADH2 gene Arg47His mutations,Arg/Arg,Arg/Hisand His/His were significantly different between the CHB Group and the Control Group（13.1%,48.4% and 38.5% vs.7.2%,42.6% and 50.2;P=0.014）.Arg and His allele frequencies in the mutated gene were also significantly different between the two groups（37.3% and 62.7% vs.28.5% and 71.5%;P=0.005）.The wild type gene and allele frequency in the CHB Group was significantly higher than in the Control Group.With the His/His genotype as a reference group,the OR values of Arg/His and Arg/Arg were 1.48（95% CI：1.01-2.17）and 2.38（95% CI：1.24-4.59）,respectively.After considering gender,age,smoker/nonsmoker,alcoholic/non-alcohokic,and with/without hepatitis family history,the OR values were adjusted to 1.46（95% CI：0.97-2.19） and 2.51（95% CI：1.25-5.02）,respectively.Conclusion The wild-type ADH2 Arg47His mutation may be one of the pathogenetic risk factors for chronic hepatitis among Chinese Han populations.

关 键 词：肝炎 乙型 慢性 基因 突变 相互作用 

分 类 号：R575.3[医药卫生—消化系统]