高效毛细管电泳法检测鸡肉中青霉素类药物残留量  

High Performance Capillary Electrophoretic Determination of Residual Amounts of Penicillin Drugs in Chicken

作　　者：陈玎玎[1] 孟欢欢[2] 祁克宗[2] 何东旭[2] 

机构地区：[1]安徽农业大学生物技术中心,合肥230036 [2]安徽农业大学动物科技学院,合肥230036

出　　处：《理化检验（化学分册）》2011年第11期1293-1295,1298,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金："十一五国家科技支撑计划"分课题(2006BAK02A08-4)

摘　　要：用4种不同的样品前处理方法,即①磷酸二氢钠萃取法(SE)、②磷酸二氢钠萃取-固相萃取法(SE-SPE)、③基质固相分散萃取法(MSPDE)和④基质固相分散萃取-固相萃取法(MSPDE-SPE)对鸡肉样品做前处理,用高效毛细管电泳测定试液中青霉素、氨苄青霉素和阿莫西林等3种药物的残留量。回收试验和色谱分离结果表明:方法②用于高效毛细管电泳法同时测定鸡肉样品中3种青霉素类药物的样品前处理更为合适。方法的加标回收率在73.1%～95.9%之间,相对标准偏差(n=5)在2.9%～9.8%之间。Chicken samples were pretreated by the following 4 methods separately： ① NaH2PO4-extraction（SE）,② NaH2PO4-solid phase extraction（SE-SPE）,③ matrix solid-phase dispersion extraction（MSPDE）and ④ matrix solid-phase dispersion extraction-SPE（MSPDE-SPE）.The contents of penicillin,ampicillin and amoxicillin in the sample solutions were determined by high performance capillary electrophoresis（HPCE）.As shown by the results obtained by recovery tests and chromatographic separation,the pretreatment method ② was more suitable to be used for simultaneous determination of the 3 penicillin drugs.Tests for recovery and precision of the proposed method were tested,values of recovery and RSD′s（n=5） found were in the ranges of 73.1%-95.9% and 2.9%-9.8% respectively.

关 键 词：样品前处理方法 高效毛细管电泳法 鸡肉 青霉素 氨苄青霉素 阿莫西林 

分 类 号：O657.63[理学—分析化学]