蜂胶黄酮的超声波提取工艺研究  

The Ultrasonic Extraction Methods of Total Flavonoids in Propolis

作　　者：潘秋月[1] 李英华[1] 周晓红[1] 杨凤杰[1] 

机构地区：[1]浙江经贸职业技术学院,浙江杭州310018

出　　处：《蜜蜂杂志》2011年第12期10-12,共3页Journal of Bee

基　　金：浙江省新苗人才项目(2010R441001);蜂产业共性关键技术研究与集成应用(2010GA701004)

摘　　要：通过单因素实验研究了蜂胶黄酮的超声波提取工艺,并对蜂胶中黄酮类化合物的清除活性氧自由基的能力进行测定。结果表明蜂胶黄酮的最佳提取工艺为:75%乙醇作为溶剂,料液比为1:10,提取时间为20 min,超声波提取2次,温度为60℃,此时黄酮提取含量最高,达到5.046 mg/g。蜂胶中黄酮提取液稀释100倍后对DPPH活性氧自由基的清除能力和Vc的清除能力相当,蜂胶黄酮是比较有开发潜力的抗氧化剂。To study the ultrasonic conditions and moni- tor the content of flavonoids in propolis, the monothetic experimental method was used to determine the best condi- tions for extraction of flavonoids in propolis by using ul- trasonic. The anti-oxidant activity was determined by us- ing DPPH. The optimum extract of total flavonoids was ob- tained under the conditions of 60℃, ratio of propolis to solvent 1：10, ethanol concentration 75% and ultrasonic ex- tracting for 20min, continuous 2 time. With the optimized technology, the content of total flavonoids in the final product was 5.046mg/g. Extraction of propolis flavonoids dilution （multiple 100 diluted solution） has the same an- ti-oxidant activity with the same concentration VC. Propo- lis has a bright future for antioxidant exploitation.

关 键 词：蜂胶 黄酮 超声波提取 抗氧化 DPPH自由基 

分 类 号：TQ461[化学工程—制药化工]