肠毒素源性定居因子CS6在减毒伤寒沙门氏菌中表达及免疫原性的研究  

STABLE EXPRESSION AND IMMUNOGENICITY IN A ATTENUATED SALMONELLA TYPHI STRAIN OF COLI SURFACE ANTIGEN 6 OF ENTEROTOXIGENIC ESCHERICHIA COLI 

作　　者：滕家波[1] 芮贤良[2] 张毅[3] 张兆山[2] 苏国富[2] 

机构地区：[1]卫生部长春生物制品研究所,长春130062 [2]军事医学科学院生物工程研究所,北京100071 [3]白求恩医科大学,长春130021

出　　处：《微生物学报》1999年第6期533-538,共6页Acta Microbiologica Sinica

基　　金：国家自然科学基金

摘　　要：将编码肠毒素源性大肠杆菌定居因子抗原ＣＳ６ 基因克隆至ｐＸＬ６７０ ，转化ａｓｄ 基因突变的Ｅ．ｃｏｌｉ Ｘ６０９７ ，获得重组质粒ｐＳＳ６４ ，再将后者转化至减毒的△ａｒｏＡ、△ａｒｏＣ、△ａｓｄ 伤寒沙门氏菌，构建了无药物抗性且稳定的大肠杆菌和伤寒双价菌苗候选株。小鼠腹腔免疫和攻击实验表明，该菌株对伤寒沙门氏菌毒株的攻击具有良好的保护作用。家兔免疫实验证明，该菌株能产生抗ＣＳ６ 和伤寒菌ＶｉA gene fragment encoding for surface antigen CS6 of enterotoxigenic of Escherichia coli has been cloned into the plasmid pXL670, a new recombinant plasmid pSS64 was obtained by transforming E.coli X6097 with asd gene deletion. A safe and effective E.coli/S.typhi bivalent candidate vaccine was constructed by introducing pSS64 into △aroA、△aroC、△asd Salmonella typhi. The vaccine strain is still stable in the absence of antibiotics. Animal tests demonstrated that this strain, when administered subcutaneously in mice, could provide significant protection against the intraperitoneal challenge from wild S.typhi Ty2. Immunization of rabbit with this strain raised specific antibody responses against CS6 and Vi antigen of S.typhi . This study lays the foundation for the construction of a new E.coli/S.typhi bivalent live oral vaccine.

关 键 词：定居因子CS6 伤寒沙门氏菌 双价活菌苗 ETEC 

分 类 号：R392－33[医药卫生—免疫学] R394.8[医药卫生—基础医学]